Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination

作     者：Yu-qin MEI Song-quan SONG 

作者机构：Institute of Botany Chinese Academy of Sciences Beijing 100093 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2010年第11卷第12期

页      面：965-972页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 

基　　金：Project (No. 30870223) supported by the National Natural Science Foundation of China 

主　　题：大麦种子 跨忍耐 萌芽 / 生长 清除的反应的氧种类(ROS ) 酶 在低温度的预告的处理 温度应力 

摘      要：A number of studies have shown the existence of cross-tolerance in plants,but the physiological mechanism is poorly *** this study,we used the germination of barley seeds as a system to investigate the cross-tolerance of low-temperature pretreatment to high-temperature stress and the possible involvement of reactive oxygen species (ROS) scavenging enzymes in the *** pretreatment at 0 °C for different periods of time,barley seeds were germinated at 35 °C,and the content of malondialdehyde (MDA) and the activities of ROS scavenging enzymes were measured by a spectrophotometer *** results showed that barley seed germinated very poorly at 35 °C,and this inhibitive effect could be overcome by pretreatment at 0 °*** MDA content varied,depending on the temperature at which seeds germinated,while barley seeds pretreated at 0 °C did not change the MDA *** with seeds germinated directly at 35 °C,the seeds pretreated first at 0 °C and then germinated at 35 °C had markedly increased activities of superoxide dismutase (SOD),ascorbate peroxidase (APX),catalase (CAT),and glutathione reductase (GR).The SOD and APX activities of seeds germinated at 35 °C after 0 °C-pretreatment were even substantially higher than those at 25 °C,and GR activity was similar to that at 25 °C,at which the highest germination performance of barley seeds was *** results indicate that low-temperature pretreatment can markedly increase the tolerance of barley seed to high temperature during germination,this being related to the increase in ROS scavenging enzyme *** may provide a new method for increasing seed germination under stress environments,and may be an excellent model system for the study of cross-tolerance.