Elucidation and engineering mitochondrial respiratory-related genes for improving bioethanol production at high temperature in Saccharomyces cerevisiae

作     者：Xianni Qi Zhen Wang Yuping Lin Yufeng Guo Zongjie Dai Qinhong Wang 

作者机构：Key Laboratory of Engineering Biology for Low-carbon BiosynthesisTianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China National Center of Technology Innovation for Synthetic BiologyTianjin 300308China College of Science&TechnologyHebei Agricultural UniversityCangzhou 061100China 

出 版 物：《Engineering Microbiology》 (工程微生物学（英文）)

年 卷 期：2024年第4卷第2期

页      面：12-23页

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：supported by the National Key Research and De-velopment Program of China (2021YFC2103300),Research Equipment Program of Chinese Academy of Sciences (YJKYYQ20170023) National Natural Science Foundation of China (32071423,31470214,and 32200067) Natural Science Foundation of Hebei Province (C2020204013) Development Program Projects of Hebei Province (22322905D) 

主　　题：Saccharomyces cerevisiae Adaptive evolution Transcriptional sequencing Mitochondrial respiratory Thermotolerance 

摘      要：Industrial manufacturing of bioproducts,especially bioethanol,can benefit from high-temperature fermentation,which requires the use of thermotolerant yeast *** activity in yeast is closely related to its over-all ***,the mitochondrial respiratory changes in response to adaptive thermotolerance are still poorly understood and have been rarely utilized for developing thermotolerant yeast cell ***,adap-tive evolution and transcriptional sequencing,as well as whole-genome-level gene knockout,were used to obtain a thermotolerant strain of Saccharomyces ***,thermotolerance and bioethanol production efficiency of the engineered strain were *** evaluation showed the boosted fermentation ca-pacity and suppressed mitochondrial respiratory activity in the thermotolerant *** improved fermentation produced an increased supply of adenosine triphosphate required for more active energy-consuming *** analysis revealed significant changes in the expression of the genes involved in the mitochondrial respiratory *** of mitochondria-associated gene knockout confirmed that ADK1,DOC1,or MET7 were the key factors for the adaptive evolution of thermotolerance in the engineered yeast ***,overexpression of DOC1 with TEF1 promoter regulation led to a 10.1%increase in ethanol production at 42℃.The relationships between thermotolerance,mitochondrial activity,and respiration were explored,and a ther-motolerant yeast strain was developed by altering the expression of mitochondrial respiration-related *** study provides a better understanding on the physiological mechanism of adaptive evolution of thermotolerance in yeast.