Quantitative detection of citrate for early stage diagnosing of prostate cancer:Discriminating normal to cancer in prostate tissues

作     者：Selvaraj Muthusamy Kanagaraj Rajalakshmi Palanisamy Kannan Yun-Sik Nam Dongwei Zhu Yeonggil Seo Jong-Won Song Dong Nyoung Heo Il Keun Kwon Yuanguo Xu Selvaraj Muthusamy;Kanagaraj Rajalakshmi;Palanisamy Kannan;Yun-Sik Nam;Dongwei Zhu;Yeonggil Seo;Jong-Won Song;Dong Nyoung Heo;Il Keun Kwon;Yuanguo Xu

作者机构：School of Chemistry and Chemical EngineeringJiangsu UniversityZhenjiang 212013China College of BiologicalChemical Sciences and EngineeringJiaxing UniversityJiaxing 314001China Advanced Analysis CenterKorea Institute of Science & TechnologySeongbuk-guSeoul02792Republic of Korea School of MedicineJiangsu UniversityZhenjiang 212013China Department of Chemistry EducationDaegu UniversityGyeongsan-siGyeongsangbuk-do 38453Republic of Korea Department of Dental MaterialsSchool of DentistryKyung Hee UniversitySeoulRepublic of Korea 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第2期

页      面：501-506页

核心收录：

学科分类：1002[医学-临床医学] 07[理学] 070302[理学-分析化学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China(No.22150410327) the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT&Future Planning(No.2020R1A2C1102741). 

主　　题：Prostate cancer Biomarker-citrate Boronic acid Lewis acid/base-ICT Red-emissive Mitochondria targeting Prostate tissue 

摘      要：Prostate cancer(PC)biomarker-citrate detection is clinically important to diagnose PC in early stages.Methylquinolinium iodide(Q)conjugated indole-phenylboronic acid(IB)was designed as a red-emissive QIB probe for the detection of citrate through Lewis acid-base reaction and intramolecular charge transfer(ICT)sensing mechanisms.Boronic acid acts as Lewis acid as well as citrate(Lewis base)recognition unit.The probe reacted with citrate,showing enhanced red emissions.Since the probe has excellent water solubility and great biocompatibility,practical application in biological systems is possible.Citrate was monitored precisely in the mitochondria organelle(in vitro)of living cells with a positive charge on QIB.Also,endogenous(in situ)citrate was detected quantitatively to discriminate non-cancerous and PC mice,observed strong and lower(negligible)emission intensity on non-cancerous and cancerous prostate tissues,respectively.Because,the concentration of citrate is higher in healthy prostate compared with PC prostate.Furthermore,the analysis of sliced prostate tissues can give PC-related information for clinical diagnosis to prevent and treat PC in the initial stages.Therefore,we believe that the present probe is a promising biochemical reagent in diagnosing PC.