Tumor Receptor-Seeking Dyes for Rapid Intraoperative Definition of Tumor Margin and Histopathological Morphology

作     者：Yajun Wang Feiran Zhang Yunlong Jia Tianyang Han Chengbin Zhang Limei Qu Yuewei Zhang Shoujun Zhu 

作者机构：State Key Laboratory of Supramolecular Structure and MaterialsCenter for Supramolecular Chemical BiologyCollege of ChemistryJilin UniversityChangchun 130012 Joint Laboratory of Opto-Functional Theranostics in Medicine and ChemistryThe First Hospital of Jilin UniversityChangchun 130021 School of Chemistry and Pharmaceutical EngineeringJilin Institute of Chemical TechnologyJilin 132022 Department of PathologyThe First Hospital of Jilin UniversityChangchun 130021 

出 版 物：《CCS Chemistry》 (中国化学会会刊（英文）)

年 卷 期：2024年第6卷第10期

页      面：2577-2593页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Key Research and Development Program of China(grant no.2022YFC2601900) 

主　　题：tumor receptor-seeking dyes tumor-positive margins chemoselective covalent labeling nearinfrared imaging histopathological morphology 

摘      要：Histopathological diagnosis is a crucial part of surgical treatment,as it determines further treatment decisions and prognosis for *** to the heterogeneity of tumors,traditional histopathological diagnosis relies on histological and cytological morphological analysis of cryo-slides by well-trained pathologists,which is subjective,time-consuming,and lacks considerable ***,we engineer a series of tumor receptor-seeking dyes to automatically label and visually depict the tumor contour with antibody-free *** meso-chlorine on the cyclohexyl ring of dyes can covalently react with specific thiol groups in various tumor signature proteins to form stable dye@protein *** dye@protein complexes have significantly amplified fluorescence signals in tumor sites,enabling highresolution delineation of tumormargins and providing histological morphology information to potentially determine tumor *** orthogonal effect not only avoids errors caused by individual signature proteins,but also amplifies the fluorescence difference between tumor and normal ***,our method is easy to operate with a minimal detection time of only 4 *** tumor receptor-seeking dyes successfully assess tumor-positive margins from 66 resected breast tumor samples and 25 intraoperative cancer patients with 100%accuracy,providing a newgeneration technique for intraoperative pathologic diagnosis of tumor biopsy.