Role of integrin b1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel

作     者：Yuanjun Huang Miao Sun Zhenhui Lu Qiuling Zhong Manli Tan Qingjun Wei Li Zheng 

作者机构：Guangxi Engineering Center in Biomedical Materials for Tissue and Organ RegenerationThe First Affiliated Hospital of Guangxi Medical UniversityGuangxi Medical UniversityNanning 530021China Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-Constructed by the Province and MinistryFirst Affiliated Hospital of Guangxi Medical UniversityGuangxi Medical UniversityNanning 530021China Department of Trauma Orthopedic and Hand SurgeryThe First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Guangxi Key Laboratory of Regenerative MedicineThe First Affiliated Hospital of Guangxi Medical UniversityGuangxi Medical UniversityNanning 530021China Life Science InstituteGuangxi Medical UniversityNanning 530021China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2024年第3卷第5期

页      面：59-73页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by the Guangxi Scientific Research and Technological Development Foundation(grant number GuikeAB23026049) Guangxi Natural Science Foundation(grant number 2023GXNSFBA026034) the National Natural Science Foundation of China(grant number 82360426) 

主　　题：collagen hydrogel chondrogenic induction integrin b1 tenascin C TGF-SMAD2/3 signaling 

摘      要：Cartilage defects may lead to severe degenerative joint *** engineering based on type I collagen hydrogel that has chondrogenic potential is ideal for cartilage ***,the underlying mechanisms of chondrogenic differentiation driven by type I collagen hydrogel have not been fully ***,we explored potential collagen receptors and chondrogenic signaling pathways through bioinformatical analysis to investigate the mechanism of collagen-induced *** showed that the super enhancer-related genes induced by collagen hydrogel were significantly enriched in the TGF-b signaling pathway,and integrin-b1(ITGB1),a receptor of collagen,was highly expressed in bone marrow mesenchymal stem cells(BMSCs).Further analysis showed genes such as COL2A1 and Tenascin C(TNC)that interacted with ITGB1 were significantly enriched in extracellular matrix(ECM)structural constituents in the chondrogenic induction *** of ITGB1 led to the downregulation of cartilage-specific genes(SOX9,ACAN,COL2A1),SMAD2 and TNC,as well as the downregulation of phosphorylation of SMAD2/*** of TNC also resulted in the decrease of cartilage markers,ITGB1 and the SMAD2/3 phosphorylation but overexpression of TNC showed the opposite ***,in vitro and in vivo experiments confirmed the involvement of ITGB1 and TNC in collagen-mediated chondrogenic differentiation and cartilage *** summary,we demonstrated that ITGB1 was a crucial receptor for chondrogenic differentiation of BMSCs induced by collagen *** can activate TGF-SMAD2/3 signaling,followed by impacting TNC expression,which in turn promotes the interaction of ITGB1 and TGF-SMAD2/3 signaling to enhance *** may provide concernful support for cartilage tissue engineering and biomaterials development.