Fasting as a precursor to high-fat diet enhances mitochondrial resilience in Drosophila melanogaster

作     者：Florence Hunter-Manseau Simon BCormier Rebekah Strang and Nicolas Pichaud 

作者机构：Department of Chemistry and BiochemistryUniversitéde MonctonMonctonNew BrunswickCanada New Brunswick Centre for Precision MedicineMonctonNew BrunswickCanada 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第6期

页      面：1770-1788页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by grants from the Natural Sciences and Engineering Research Council of Canada(NSERC)(grant number RGPIN-2017-05100 and RGPIN-2023-05945) the Universitéde Moncton the New Brunswick Innovation Foundation(NBIF)to NP(Emerging Project and Research Assistantship Initiative) supported by a NSERC doctoral scholarship(PGS D) 

主　　题：dietary modulation fasting high-fat diet metabolic flexibility mitochondrial metabolism stress response 

摘      要：Changes in diet type and nutrient availability can impose significant environmental stress on organisms,potentially compromising physiological functions and reproductive *** nature,dramatic fluctuations in dietary resources are often observed and adjustments to restore cellular homeostasis are crucial to survive this type of *** this study,we exposed male Drosophila melanogaster to two modulated dietary treatments:one without a fasting period before exposure to a high-fat diet and the other with a 24-h fasting *** then investigated mitochondrial metabolism and molecular responses to these *** to a high-fat diet without a preceding fasting period resulted in disrupted mitochondrial respiration,notably at the level of complex *** the other hand,a short fasting period before the high-fat diet maintained mitochondrial ***,transcript abundance of genes associated with mitophagy,heatshock proteins,mitochondrial biogenesis,and nutrient sensing pathways increased either slightly or significantly following a fasting period and remained stable when flies were subsequently put on a high-fat diet,whereas a drastic decrease of almost all transcript abundances was observed for all these pathways when flies were exposed directly to a high-fat ***,mitochondrial enzymatic activities showed less variation after the fasting period than the treatment without a fasting ***,our study sheds light on the mechanistic protective effects of fasting prior to a high-fat diet and highlights the metabolic flexibility of Drosophila mitochondria in response to abrupt dietary changes and have implication for adaptation of species to their changing environment.