The Study on Improved Cryopreservation Technique of The Ultrastructure of Corneal Endothelial Cells

作     者：Zhiyang Jia, Shujin Liang, Changling Wang , Peixin ZhugeDepartment of Ophthalmology, The Second Affiliated Hospital of Hebei Medical College Shijiazhuang 050000, China.Laboratory of Eye Disease, The Second Affiliated Hospital of Hebei Medical college Shijiazhuang 050000, China 

作者机构：Department of Ophthalmology Second Affiliated Hospital of Hebei Medical College Shijiazhuang China. 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：1994年第10卷第1期

页      面：13-20页

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

主　　题：corneal endothelium ultrastructure cryopreservation 

摘      要：The traditional corneal cryopreservation technique was improved. We carried out an experimental study that rabbit corneas were cryop-reserved by using polyvinylpyrolidone (PVP) as cryoprotective agent and dimethlsulfoxide (DMSO) as the control. The endothelia of cryopreserved corneas were evaluated by scanning and transmission electron microscopy and vital staining. The study shows that PVP is an excellent extracellular cryoprotective agent and has the characteristic of low toxicity or no toxicity to co...