Data driven analysis reveals prognostic genes and immunological targets in human sepsis-associated acute kidney injury

作     者：Qing Zhao Jinfu Ma Jianguo Xiao Zhe Feng Hui Liu Qing Zhao;Jinfu Ma;Jianguo Xiao;Zhe Feng;Hui Liu

作者机构：Department of Diagnosis and Treatment of Cadresthe First Medical CenterChinese PLA General HospitalBeijing 100853China Intensive Care Unitthe 305th Hospital of Chinese PLABeijing 100032China Department of Critical Care Medicinethe First Medical CenterChinese PLA General HospitalBeijing 100853China Nephrology Departmentthe First Medical CenterChinese PLA General HospitalBeijing 100853China 

出 版 物：《World Journal of Emergency Medicine》 (世界急诊医学杂志（英文）)

年 卷 期：2024年第15卷第2期

页      面：91-97页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Beijing Natural Science Foundation(No.7222162 to Dr.Hui Liu) 

主　　题：Sepsis Acute kidney injury Interleukin 32 Toll-like receptor 2 Bioinformatics analysis 

摘      要：BACKGROUND:The molecular mechanism of sepsis-associated acute kidney injury(SA-AKI)is *** analyzed co-differentially expressed genes(co-DEGs)to elucidate the underlying mechanism and intervention targets of ***:The microarray datasets GSE65682,GSE30718,and GSE174220 were downloaded from the Gene Expression Omnibus(GEO)*** identified the co-DEGs and constructed a gene co-expression network to screen the hub *** analyzed immune correlations and disease correlations and performed functional annotation of the hub *** also performed single-cell and microenvironment analyses and investigated the enrichment pathways and the main transcription ***,we conducted a correlation analysis to evaluate the role of the hub ***:Interleukin 32(IL32)was identified as the hub gene in SA-AKI,and the main enriched signaling pathways were associated with hemopoiesis,cellular response to cytokine stimulus,inflammatory response,and regulation of kidney ***,IL32 was significantly associated with mortality in SA-AKI ***,macrophages,T cells,and NK cells were closely related to IL32 and were involved in the immune microenvironment in SA-AKI ***32 expression increased significantly in the kidney of septic ***-like receptor 2(TLR2)was significantly and negatively correlated with ***:IL32 is the key gene involved in SA-AKI and is significantly associated with ***2 and relevant immune cells are closely related to key genes.