Chick chorioallantoic membrane model to investigate role of migrasome in angiogenensis
作者机构：The State Key Laboratory of Membrane Biology Tsinghua University-Peking University Joint Centre for Life Sciences Beijing Frontier Research Center for Biological Structure Xiamen Cardiovascular Hospital Xiamen University
出 版 物：《Biophysics Reports》 (生物物理学报)
年 卷 期：2023年第9卷第5期
页 面：241-254页
核心收录：
学科分类：07[理学]
基 金：supported by the Ministry of Science and Technology of the People’s Republic of China (grant no.2017YFA0503404) the National Natural Science Foundation of China (31790401, 92054301 and 32030023) the Beijing Municipal Science&Technology Commission(Z201100005320019 and Z211100003321002) internal grant from Tsinghua university (2030 plan)
主 题：Angiogenesis Chorioallantoic membrane (CAM) Chick CAM model
摘 要：The development of the vascular system is essential for embryonic development, including processes such as angiogenesis. Angiogenesis plays a critical role in many normal physiological and pathological processes. It is driven by a set of angiogenic proteins, including angiogenic growth factors, chemokines,and extracellular matrix proteins. Among various animal model systems, the chorioallantoic membrane(CAM), a specialized and highly vascularized tissue of the avian embryo, has proven to be a valuable tool for analyzing the angiogenic potential of candidate cells or factors. In this protocol, we provide detailed procedures for establishing the CAM model to evaluate the function and mechanism of migrasomes in embryonic angiogenesis. This includes the CAM nylon mesh assay and CAM ex vivo sprouting assay to assess CAM angiogenesis, as well as the observation, purification, and delivery of migrasomes. Additionally, we describe the generation of T4-KO-m Cherry-KI embryos using the CRISPR system within the CAM tissue to investigate the role of migrasomes in angiogenesis.
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