MicroR-9c-5p and novel-mir50 co-target Akt to regulate Lasioderma serricorne reproduction
作者机构：Provincial Key Laboratory for Agricultural Pest Management of Mountainous RegionInstitute of EntomologyGuizhou UniversityGuiyangChina Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous RegionCollege of Biology and Environmental EngineeringGuiyang UniversityGuiyangChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2024年第31卷第1期
页 面：106-118页
核心收录：
基 金：This research was funded by the National Natural Science Foundation of China(32160637) the Project of China Tobacco Guizhou Industrial Co.,Ltd.(2017-25) the Special Project for Local(Guizhou)Science and Technology Development under the guidance of the Central Government(QKZYD4013) the Graduate Research Project of Guizhou Province(QJHYJSKYJJ194) the Program of Excellent Innovation Talents in Guizhou Province(20206003).
主 题：Akt insulin Lasioderma serricorne microRNA reproduction
摘 要：High fecundity is a common characteristic of insect pests which increases the difficulty of population control.Serine/threonine kinase Akt is an indispensable component of the insulin signaling pathway.Silencing of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect pest.However,the post-transcriptional pathway of LsAkt in L.serricorne remains unknown.This study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of LsAkt.The expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult stages.Luciferase reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of LsAkt.Overexpression of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian development.It also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of Akt.Conversely,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian development.Injection of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt expression.It also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin signaling.These results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of L.serricorne by targeting Akt in response to insulin signaling.The data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.
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