Common Artifacts and Remedies in Histological Preparations
Common Artifacts and Remedies in Histological Preparations作者机构：College of Medicine University of Sumer Al-Rifai Iraq
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2024年第15卷第3期
页 面：174-183页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Artifacts Histological Preparations
摘 要：The importance of the accuracy of preparing biological specimen as histological sections that can be examined under a microscope lies in reflecting a true image of the tissue that includes all its components, which are used in scientific research or for the purpose of diagnosing various diseases of the body. Despite this, some cellular structures within the tissue may suffer from some alterations that result from the appearance of defects during any stage of preparing these microscopic sections, which alter or interfere with the precise cellular structures and morphology that constitute the tissue and thus give a different image for tissue features and cause confusion in the work histopathologist in the diagnosis. There are several reasons that can cause a misdiagnosis of the sample that occurs during the surgical separation process or after separation during the stages of microscopic preparation techniques from fixation stage, tissue processing, embedding or microtomy, staining until mounting procedures. The constant need to identify these defects and their causes in addition to try to reduce them is one of the biggest challenges evident in pathology laboratories. Therefore, this study aims to review the most common defects that occur in any stage of tissue processing, with an explanation of their causes and appropriate ways to avoid them.
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