ChMYB1-ChbHLH42-ChTTG1 module regulates abscisic acidinduced anthocyanin biosynthesis in Cerasus humilis

作     者：Xiaolong Ji Zhe Li Mingyu Zhang Shaoyu Lang Xingshun Song Xiaolong Ji;Zhe Li;Mingyu Zhang;Shaoyu Lang;Xingshun Song

作者机构：State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinHeilongjiang 150040China Department of GeneticsCollege of Life ScienceNortheast Forestry UniversityHarbinHeilongjiang 150040China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第1期

页      面：51-65页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：funded by the National Natural Science Foundation of China(Grant No.32171737) the Innovation Project of State Key Laboratory of Tree Genetics and Breeding(Northeast Forestry University)(Grant No.2021A04) Natural Science Foundation of Heilongjiang Province(Grant No.LH2021C007) the Fundamental Research Funds for the Central Universities(Grant No.2572021DX07)。 

主　　题：Cerasus humilis Anthocyanin ChMYB1 Fruit ABA 

摘      要：Cerasus humilis is a kind of economic fruit tree peculiar to China,which is widely used in the food,landscape,and pharmaceutical industries.Anthocyanins are a phenolic metabolite that plays an essential role in fruit coloration.However,the regulatory network of C.humilis in anthocyanin biosynthesis is still unclear.In this study,the R2R3-MYB transcription factor ChMYB1 was isolated from the full genome of the species.Yeast one-hybrid,dual-luciferase assays,and GUS staining showed that ChMYB1 significantly increased anthocyanin contents in C.humilis fruit by promoting the expression of ChCHS and ChUFGT by binding MBS(MYB-binding elements).ChMYB1 interacted with ChbHLH42and ChTTG1 to form the MBW complex and further enhanced the expression of ChUFGT.In addition,abscisic acid(ABA)treatment promoted the expression of ChMYB1 and anthocyanin accumulation in C.humilis fruit.Interestingly,ABA treatment enhanced the interaction between ChMYB1 and ChbHLH42.Furthermore,ChABI5 inhibited the interaction between ChMYB1 and ChbHLH42.Our data elucidated the primary molecular mechanism of anthocyanin biosynthesis in C.humilis fruit,deepening the understanding of the regulatory network affecting anthocyanin metabolism in edible fruit crops.