Geobacter sulfurreducens promoted the biosynthesis of reduced graphene oxide and coupled it for nitrobenzene reduction

作     者：Shoujuan Zhang Qi Liu Linrui Zhong Jianhong Jiang Xiaozhe Luo Xingxin Hu Qian Liu Yue Lu Shoujuan Zhang;Qi Liu;Linrui Zhong;Jianhong Jiang;Xiaozhe Luo;Xingxin Hu;Qian Liu;Yue Lu

作者机构：College of Environmental Science and EngineeringHunan UniversityChangsha 410082China Shenzhen Research InstituteHunan UniversityShenzhen 510082China Key Laboratory of Environment Biology and Pollution ControlHunan UniversityMinistry of EducationChangsha 410082China China Machinery International Engineering Design&Research Institute Co.LtdChangsha 410007China Hunan Engineering Research Center for Water Treatment Process&EquipmentChangsha 410007China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2024年第138卷第4期

页      面：458-469页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

基　　金：supported by the Science and Technology Innovation Program of Hunan Province(No.2022RC1026) Shenzhen Science and Technology Program(No.JCYJ20220530160412027) Guangdong Basic and Applied Basic Research Foundation(No.2023A1515011807) the Project of the National Key Research and Development Program of China(No.2021YFC1910400) the Technical Innovation Leading Plan Project for Hunan High-tech Industry(Nos.2020SK2042 and 2022GK4062) the Key R&D Project of Hunan Province of China(No.2022SK2067)。 

主　　题：Reduced graphene oxide Geobacter Nitrobenzene Removal Extracellular electron transfer 

摘      要：In order to explore an efficient and green method to deal with nitrobenzene(NB)pollutant,reduced graphene oxide(r GO)as an electron shuttle was applied to enhance the extracellular electron transfer(EET)process of Geobacter sulfurreducens,which was a typical electrochemically active bacteria(EAB).In this study,r GO biosynthesis was achieved via the reduction of graphene oxide(GO)by G.sulfurreducens PCA within 3 days.Also,the r GOPCA combining system completely reduced 50-200μmol/L of NB to aniline as end product within one day.SEM characterization revealed that PCA cells were partly wrapped by rGO,and therefore the distance of electron transfer between strain PCA and r GO material was reduced.Beside,the ID/IGof GO,r GO,and r GO-PCA combining system were 0.990,1.293 and 1.31,respectively.Moreover,highest currents were observed in r GO-PCA-NB as 12.950μA/-12.560μA at -408 m V/156 m V,attributing to the faster electron transfer efficiency in EET process.Therefore,the NB reduction was mainly due to:(I)direct EET process from G.sulfurreducens PCA to NB;(II)r GO served as electron shuttle and accelerated electron transfer to NB,which was the main degradation pathway.Overall,the biosynthesis of r GO via GO reduction by Geobacter promoted the NB removal process,which provided a facile strategy to alleviate the problematic nitroaromatic pollution in the environment.