Construction of algal-bacterial consortia using green microalgae Chlorella vulgaris and As(Ⅲ)-oxidizing bacteria:As tolerance and metabolomic profiling

作     者：Xiaoman He Guobing Lin Jiayuan Zeng Zhaoguang Yang Lin Wang Xiaoman He;Guobing Lin;Jiayuan Zeng;Zhaoguang Yang;Lin Wang

作者机构：College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China Key Laboratory of Hunan Province for Water Environment and Agriculture Product SafetyCentral South UniversityChangsha 410083China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2024年第139卷第5期

页      面：258-266页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0815[工学-水利工程] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(No.41977351) the Natural Science Foundation of Hunan Province,China(No.2020JJ4698) 

主　　题：Microalgae Algal-bacterial consortia As(Ⅲ)-oxidizing bacteria Metabolomics Energy metabolism 

摘      要：Bioremediation became a promising technology to resolve arsenic(As)contamination in aquatic *** monoculture such as microalgae or bacteria was sensitive to environmental disturbance and vulnerable to contamination,green microalgae Chlorella vulgaris and arsenite(As(Ⅲ))-oxidizing bacteria Pseudomonas ***11 were co-cultured to construct algal-bacterial consortia in the current *** effects of algae-bacteria(A:B)ratio and exposure As(Ⅲ)concentration on algal growth,As speciation and metabolomic profile were *** growth arrested when treated with 100 mg/L As(Ⅲ)without the co-cultured *** contrast,co-cultured with strain SMS11 significantly enhanced As tolerance in *** especially with A:B ratio of 1:*** the As(Ⅲ)in culture media of the consortia were oxidized into As(Ⅴ)on day *** of As was observed on day *** 1% and 0.5% of total As were converted into dimethylarsinic acid(DMA)after 21days cultivation when the initial concentrations of As(Ⅲ)were 1 and 10 mg/L,*** analysis was further performed to reveal the response of consortia metabolites to external As(Ⅲ).The enriched metabolomic pathways were associated with carbohydrate,amino acid and energy *** acid cycle and glyoxylate and dicarboxylate metabolism were upregulated under As stress due to their biological functions on alleviating oxidative stress and protecting *** carbohydrate and amino acid metabolisms provided precursors and potential substrates for energy production and cell protection under abiotic *** of the pathways relevant to carbohydrate or amino acid metabolism were triggered by energy requirement.