Neural differentiation of human placenta-derived mesenchymal stem cells following neural cell co-culture

作     者：Nailong Yang Hongyan Zhang Xiaojuan Sun Lili Xu 

作者机构：VIP Department Medical School Hospital Qingdao University Qingdao 266003 Shandong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第1期

页      面：23-28页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071006[理学-神经生物学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：human placenta-derived mesenchymal stem cells Transwell co-culture differentiation neural cells 

摘      要：We induced human placenta-derived mesenchymal stem cells （hPMSCs） to differentiate into neural cells by adding chemical reagents, despite the fact that toxic chemicals induce cell shrinkage or cytoskeletal formation, which does not represent a proper cell differentiation process. The present study established a co-culture system with hPMSCs and neural cells and analyzed the influence of neural cells on hPMSC differentiation in a co-culture system, hPMSCs were isolated and purified from human full-term placenta using collagenase digestion. Fetal neural cells were co-cultured with hPMSCs for 48 hours using the Transwell co-culture system, hPMSCs co-cultured with neural cells exhibited a slender morphology with a filament. After 96 hours, hPMSCs expressed neuron-specific enolase, which suggested that co-culture of hPMSCs and neural cells induced neural differentiation of hPMSCs.