μ-and m-calpain expression and activity changes following diethylstilbestrol injection in the rat anterior pituitary

作     者：Weijiang Zhao Zhongfang Shi Fang Yuan Guilin Li Yazhuo Zhang Zhongcheng Wang 

作者机构：Neuroscience Center Shantou University Medical College Shantou 51504 I Guangdong Province China Beijing Neurosurgical Institute Capital Medical University Beijing 100050 China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第1期

页      面：60-65页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 0832[工学-食品科学与工程（可授工学、农学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 09[农学] 0906[农学-兽医学] 083203[工学-农产品加工及贮藏工程] 

主　　题：diethylstilbestrol ubiquitous calpain anterior pituitary prolactinoma Wistar rats 

摘      要：Little is known about changes in calpain activity in the pituitary gland. In the present study,μ- and m-calpain activity changes were detected in the rat anterior pituitary following intraperitoneal injection of diethylstilbestrol. Double-immunofluorescence labeling confirmed colocalization of μ- and m-calpain in prolactin-secreting cells （lactotrophs）. Western blot analysis revealed significantly increased expression of both calpains, which accompanied upregulated cytosol and membrane zymographic activities at 12 weeks following diethylstilbestrol injection, compared with rats injected with sunflower oil. Moreover, following estrogen injection, pituitary gland pathological damage gradually worsened with increasing time. Results demonstrated that estrogen regulated calpain expression and activity, and both calpains participated in the pathophysiological processes of the pituitary gland. Ubiquitous calpain expression could serve as an effective target for anti-estrogen drugs.