含有FXa切割位点的抗菌肽X在大肠杆菌中的融合表达

作     者：袁榴娣 窦非 梁玉璞 谢维 王芳 张双全 戴祝英 YUAN Liu-Di;DOU Fei;LIANG Yu-Pu;XIE Wei;WANG Fang;ZHANG Shuang-quan;DAI Zhu-ying

作者机构：南京大学生物化学系南京大学医药生物技术国家重点实验室南京210093 南京铁道医学院基础医学系南京210009 南京师范大学生物系南京210008 

出 版 物：《生物工程学报》 (Chinese Journal of Biotechnology)

年 卷 期：2000年第16卷第3期

页      面：411-414页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：国家自然科学基金!( 3 95 70 111) 铁道部科学和技术基金!( 96z0 61)资助项目 

主　　题：抗菌肽X 融合表达 凝血因子FXa 抗菌活性 

摘      要：PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X,then the gene was cloned into the expression vector pGEX KG,and was highly expressed in *** BL21 by IPTG *** fusion protein was purified by affinity chromatography and was cleaved by Factor *** X with antibacterial activity was obtained after purified by ion exchange chromatography.