深低温保存下高效抗冻多肽的合理设计和机理探讨

作     者：Haishan Qi Yihang Gao Lin Zhang Zhongxin Cui Xiaojie Sui Jianfan Ma Jing Yang Zhiquan Shu Lei Zhang Haishan Qi;Yihang Gao;Lin Zhang;Zhongxin Cui;Xiaojie Sui;Jianfan Ma;Jing Yang;Zhiquan Shu;Lei Zhang

作者机构：Department of Biochemical EngineeringSchool of Chemical Engineering and TechnologyFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE)Tianjin UniversityTianjin 300350China School of Engineering and TechnologyUniversity of Washington TacomaTacomaWA 98402USA 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2024年第34卷第3期

页      面：164-173页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Key Research and Development Program of China (2021YFC2100800) the National Natural Science Foundation of China (22078238,21961132005,and 21908160) the Open Funding Project of the National Key Laboratory of Biochemical Engineering the Program of Introducing Talents of Discipline to Universities (BP0618007) 

主　　题：Antifreeze peptides Evolution analysis Ice recrystallization inhibition Molecular dynamics simulation Cryopreservation Synthetic biology 

摘      要：The development of effective antifreeze peptides to control ice growth has attracted a significant amount of attention yet still remains a great ***,we propose a novel design method based on in-depth investigation of repetitive motifs in various ice-binding proteins(IBPs)with evolution *** this way,several peptides with notable antifreeze activity were *** particular,a designed antifreeze peptide named AVD exhibits ideal ice recrystallization inhibition(IRI),solubility,and biocompatibility,making it suitable for use as a cryoprotective agent(CPA).A mutation analysis and molecular dynamics(MD)simulations indicated that the Thr6 and Asn8 residues of the AVD peptide are fundamental to its ice-binding capacity,while the Ser18 residue can synergistically enhance their interaction with ice,revealing the antifreeze mechanism of ***,to evaluate the cryoprotection potential of AVD,the peptide was successfully employed for the cryopreservation of various cells,which demonstrated significant post-freezing cell *** work opens up a new avenue for designing antifreeze materials and provides peptide-based functional modules for synthetic biology.