Chiral coating-mediated interactions of bacteria with diverse biointerfaces

作     者：Hejin Jiang Rui Liu Ying Liu Huilong Luo Zhenping Cao Jinyao Liu Hejin Jiang;Rui Liu;Ying Liu;Huilong Luo;Zhenping Cao;Jinyao Liu

作者机构：Shanghai Key Laboratory for Nucleic Acid Chemistry and NanomedicineInstitute of Molecular MedicineState Key Laboratory of Systems Medicine for CancerRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2023年第66卷第12期

页      面：3594-3601页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0703[理学-化学] 071005[理学-微生物学] 

基　　金：supported by the National Key Research and Development Program of China (2021YFA0909400) the National Natural Science Foundation of China (22102097,52101289,21875135) the Fundamental Research Funds for the Central Universities (2020JCPT02) the Innovative Research Team of High-Level Local Universities in Shanghai (SHSMU-ZDCX20210900) the Explorer Program of the Science and Technology Commission of Shanghai Municipality (21TS1400400) the Shanghai Municipal Education CommissionGaofeng Clinical Medicine Grant Support (20181704)。 

主　　题：bacteria coating chiral adhesion living agents 

摘      要：Bacteria,as living agents,have been widely used for microbial therapy due to their inherent ability to colonize different in vivo microenvironments,particularly in the gut and tumor.The interaction with diverse biointerfaces plays a critical role in bacterial colonization,and thereby determine the ultimate efficacy of microbial therapy.Although surface modification of bacteria with exogenous functional motifs can vary their interaction with surroundings,the effects of surface chirality of modified bacteria on mucous adhesion,tumor cell binding,and bacterial competition remain unknown.Here,we describe surface chirality-dependent selective interactions of bacteria to mucins,tumor cells,and pathogens.By coating bacteria with cationic polyethyleneimine modified with different chiral amino acids through electrostatic interaction,we find that bacteria coated with a D-chiral surface structure exhibit greatly increased adhesion to both mucins and tumor cells compared with those of L-and DL-structures.In addition,by adjusting the chirality of the coating,wrapped probiotic bacteria can selectively resist pathogenic bacteria,showing great potential to enhance colonization and positively modulate the gut and tumor microbiota.This work discloses surface chirality-dependent interaction of bacteria with different biointerfaces,showing a potential to tune the colonization and therapeutic effect of living bacterial agents for disease treatment.