NUDT5 promotes the growth,metastasis,and Warburg effect of IDH wild-type glioblastoma multiforme cells by upregulating TRIM47

作     者：Zi-Fa Zhang Shu-Ming Liu Zi-Fa Zhang;Shu-Ming Liu

作者机构：Neurosurgery DepartmentShanxi Bethune HospitalTaiyuanShanxi Province030022China Shanxi Academy of Medical SciencesTaiyuanShanxi province030022China Emergency DepartmentTaiyuan People’s HospitalTaiyuanShanxi Province030021China 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2024年第14卷第2期

页      面：82-92页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Glioblastoma multiforme NUDT5 TRIM47 Growth Metastasis Warburg effect 

摘      要：Objective:To explore the regulatory mechanism of NUDT5 in glioblastoma multiforme(GBM).Methods:GEPIA database was used to predict the expressions of NUDT5 and tripartite motif family proteins 47(TRIM47)in GBM ***-qPCR and Western blot analyses were performed to examine NUDT5 expression in GBM ***-229 cell proliferation,migration as well as invasion were estimated by CCK-8,colony formation,wound healing,and Transwell assays following interference with *** assay,L-lactic acid kit,glucose detection kit,and ATP detection kit were applied for the detection of glycolysis-related ***-immunoprecipitation experiment was carried out to verify the relationship between NUDT5 and ***:GEPIA database showed that NUDT5 expression was significantly increased in GBM *** the expression of NUDT5 in GBM cells significantly suppressed the viability,proliferation,invasion,migration,and glycolysis of GBM ***,TRIM47 was highly expressed in GBM cells and interacted with *** of TRIM47 partially reversed the inhibitory effect of NUDT5 downregulation on the proliferation,metastasis,and glycolysis of GBM ***:NUDT5 promotes the growth,metastasis,and Warburg effect of GBM cells by upregulating *** NUDT5 and TRIM47 can be used as targets for GMB treatment.