KLF4 facilitates chromatin accessibility remodeling in porcine early embryos

作     者：Wei Zhu Guowei Bu Ruifeng Hu Jixiang Zhang Lianyong Qiao Kai Zhou Tingting Wang Qiao Li Jingjing Zhang Linhui Wu Yali Xie Taotao Hu Shichun Yang Jiaqi Guan Xiaoyu Chu Juanjuan Shi Xia Zhang Falong Lu Xin Liu Yi-Liang Miao 

作者机构：Institute of Stem Cell and Regenerative BiologyCollege of Animal Science and Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Key Laboratory of Agricultural Animal GeneticsBreeding and Reproduction(Huazhong Agricultural University)Ministry of EducationWuhan 430070China State Key Laboratory of Molecular Developmental BiologyInstitute of Genetics and Developmental BiologyInnovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Frontiers Science Center for Animal Breeding and Sustainable ProductionHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan LaboratoryWuhan 430070China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2024年第67卷第1期

页      面：96-112页

核心收录：

学科分类：0710[理学-生物学] 0905[农学-畜牧学] 09[农学] 

基　　金：This work was supported by the National Natural Science Foundation of China(31902161) the National Key Research and Development Program of China(2022YFD1302201,2018YFA0107001) Strategic Priority Research Program of Chinese Academy of Sciences(XDA24020203) Key Research and Development Program of Hubei Province(2021BBA221) Major Project of Hubei Hongshan Laboratory(2021hszd003) Foundation of Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,Guizhou University(QJHKY373). 

主　　题：porcine embryos liDNase-seq chromatin accessibility KLF4 mitochondrion lipid droplets 

摘      要：Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos.Current studies have illustrated several pioneer factors as being important for agricultural animals,but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear.By employing low-input DNase-seq(liDNase-seq),we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.Our data revealed that KLF4 played critical roles in remodeling chromatin accessibility in porcine early embryos.Knocking down of KLF4 led to the reduction of chromatin accessibility in early embryos,whereas KLF4 overexpression promoted the chromatin openness in porcine blastocysts.Furthermore,KLF4 deficiency resulted in mitochondrial dysfunction and developmental failure of porcine embryos.In addition,we found that overexpression of KLF4 in blastocysts promoted lipid droplet accumulation,whereas knockdown of KLF4 disrupted this process.Taken together,our study revealed the chromatin accessibility dynamics and identified KLF4 as a key regulator in chromatin accessibility and cellular metabolism during porcine preimplantation embryo development.