Probiotic cocktails accelerate baicalin metabolism in the ileum to modulate intestinal health in broiler chickens

作     者：Mingkun Gao Chaoyong Liao Jianyang Fu Zhonghua Ning Zengpeng Lv Yuming Guo Mingkun Gao;Chaoyong Liao;Jianyang Fu;Zhonghua Ning;Zengpeng Lv;Yuming Guo

作者机构：State Key Laboratory of Animal Nutrition and FeedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2024年第15卷第2期

页      面：779-794页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：funded by National Key R&D Program of China(2022YFD1300403,2021YFD1300404) China Agriculture Research System program(CARS-40,CARS-41-G11) Beijing Natural Science Foundation(6222036) 

主　　题：Baicalin Gut health Ileal microbiota Probiotic cocktails Synergistic effects 

摘      要：Background Baicalin and probiotic cocktails are promising feed additives with broad application *** probiotic cocktails are known to enhance intestinal health,the potential synergistic impact of combining baicalin with probiotic cocktails on the gut health of broiler chickens remains largely ***,this study aims to investigate the influence of the combined administration of baicalin and probiotic cocktails on the composition of ileal and cecal microbiota in broiler chickens to elucidate the underlying mechanisms responsible for the healthpromoting *** A total of 3201-day-old male Arbor Acres broilers were divided into 4 groups,each with 8 replicates of 10 chicks per *** a period of 42 d,the birds were fed a basal diet or the same diet supplemented with 37.5 g/t baicalin(BC),1,000 g/t probiotic cocktails(PC),or a combination of both BC(37.5 g/t)and PC(1,000 g/t).The results demonstrated that BC+PC exhibited positive synergistic effects,enhancing intestinal morphology,immune function,and barrier *** was evidenced by increased VH/CD ratio,sIgA levels,and upregulated expression of occludin and claudin-1(P0.05).16S rRNA analysis indicated that PC potentiated the effects of BC,particularly in the ileum,where BC+PC significantly increased theα-diversity of the ileal microbiota,altered itsβ-diversity,and increased the relative abundance of Flavonifractor(P0.05),a flavonoid-metabolizing ***,Flavonifractor positively correlated with chicken ileum crypt depth(P0.05).While BC+PC had a limited effect on cecal microbiota structure,the PC group had a very similar microbial composition to BC+PC,suggesting that the effect of PC at the distal end of the gut overshadowed those of *** We demonstrated the synergistic enhancement of gut health regulation in broiler chickens by combining baicalin and probiotic *** cocktails enhanced the effects of baicalin and accelerated its metab