Chromosome-level genome assembly of Oncomelania hupensis: the intermediate snail host of Schistosoma japonicum

作     者：Qin Liu Lei Duan Yun-Hai Guo Li-Min Yang Yi Zhang Shi-Zhu Li Shan Lv Wei Hu Nan-Sheng Chen Xiao-Nong Zhou Qin Liu;Lei Duan;Yun-Hai Guo;Li-Min Yang;Yi Zhang;Shi-Zhu Li;Shan Lv;Wei Hu;Nan-Sheng Chen;Xiao-Nong Zhou

作者机构：National Institute of Parasitic Diseases at Chinese Center for Disease Control and Prevention(Chinese Centerfor Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector BiologyWHO Collaborating Centre for Tropical DiseasesNational Center for International Research on Tropical DiseasesNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesShanghai 200025People's Republic of China School of Global HealthChinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai 200025People's Republic of China School of Life ScienceFudan UniversityShanghai 200438People's Republic of China CAS Key Laboratory of Marine Ecology and Environmental SciencesInstitute of OceanologyChinese Academy of SciencesQingdaoShandong 266071People'sRepublicofChina 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2024年第13卷第1期

页      面：16-26页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by National Key Research and Development Program of China(No.2021YFC2300800 2021YFC2300803) 

主　　题：Schistosomiasis Schistosoma japonicum Oncomelania hupensis Chromosome-level genome 

摘      要：Background Schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis, which is a significant cause of morbidity in China, the Philippines and *** hupensis (Gastropoda: Pomatiopsidae) is the unique intermediate host ofS. japonicum. A complete genome sequence ofO. hupensis will enable the fundamental understanding of snail biology as well as its co-evolution with theS. japonicum parasite. Assembling a high-quality reference genome ofO. hupehensis will provide data for further research on the snail biology and controlling the spread ofS. *** The draft genome was de novo assembly using the long-read sequencing technology (PacBio Sequel II) and corrected with Illumina sequencing data. Then, using Hi-C sequencing data, the genome was assembled at the chromosomal level. CAFE was used to do analysis of contraction and expansion of the gene family and CodeML module in PAML was used for positive selection analysis in protein coding *** A total length of 1.46 Gb high-qualityO. hupensis genome with 17 unique full-length chromosomes (2n = 34) of the individual including a contig N50 of 1.35 Mb and a scaffold N50 of 75.08 Mb. Additionally, 95.03% of these contig sequences were anchored in 17 chromosomes. After scanning the assembled genome, a total of 30,604 protein-coding genes were predicted. Among them, 86.67% were functionally annotated. Further phylogenetic analysis revealed thatO. hupensis was separated from a common ancestor ofPomacea canaliculata andBellamya purificata approximately 170 million years ago. Comparing the genome ofO. hupensis with its most recent common ancestor, it showed 266 significantly expanded and 58 significantly contracted gene families (P 0.05). Functional enrichment of the expanded gene families indicated that they were mainly involved with intracellular, DNA-mediated transposition, DNA integration and transposase *** Integrated use of multiple sequencing technol