Analysis of differentially expressed genes in the sea cucumber Apostichopus japonicus under heat stress

作     者：Dongxue Xu Jingjing Zhang Wenqi Song Lina Sun Ji Liu Yuanxue Gu Yanru Chen Bin Xia Dongxue Xu;Jingjing Zhang;Wenqi Song;Lina Sun;Ji Liu;Yuanxue Gu;Yanru Chen;Bin Xia

作者机构：School of Marine Science and EngineeringQingdao Agricultural UniversityQingdao 266109China Key Laboratory of Mariculture of Ministry of EducationOcean University of ChinaQingdao 266003China CAS Key Laboratory of Marine Ecology and Environmental SciencesInstitute of OceanologyChinese Academy of SciencesQingdao 266071China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2023年第42卷第11期

页      面：117-126页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：The National Natural Science Foundation of China under contract Nos 42276143 and 31902360 the Shandong Provincial Natural Science Foundation under contract Nos ZR2022MC050 and ZR2022QD003 the“First Class Fishery Discipline”Programme[(2020)3]in Shandong Province 

主　　题：Apostichopus japonicus heat stress RNA-seq protein processing extracellular matrix 

摘      要：The sea cucumber Apostichopus japonicus plays important roles in marine benthic ecosystem as environmental cleaners,and it is the important aquaculture species in *** water temperature poses critical threat for the survival of ***,which has resulted in extensive death in *** explore the genes expression profiles under different levels of heat stress,the high-throughput RNA-seq was applied in this *** results revealed a total of 1371,1225 and 1408 differentially expressed genes(DEGs)in 26℃for 6 h,26℃for 48 h and 30℃for 6 h respectively in comparison with Control *** pathway analysis suggested“Protein processing in endoplasmic reticulum(ER)was significantly enriched in all these heat stress(HS)treatment *** expression results of key DEGs in this pathway(Hsp70,Derlin,NEF,PDI,GPR94 and ERP57)by qRT-PCR was in accordance with the RNA-seq *** subcluster analysis of DEGs revealed that a variety of heat shock proteins(HSPs)and calcium ion binding proteins had an obvious up-regulated expression in 26℃for 6 h,comparatively low expression in 26℃for 48 h,and the highest expression in 30℃for 6 *** other DEGs subcluster,consisting of critical components of extracellular matrix(ECM)and a subset of peptidases and proteases,showed significantly rising tendency in 30℃for 6 ***,the expression of matrix metalloproteases(MMP1,MMP16 and MMP19)was prominently affected by HS,and peaked in 30℃for 6 *** study provides a series of candidate genes for further study about heat shock response in ***,especially genes associated with protein processing in ER and regulation of ECM,which also offers new insights into cellular homeostasis under stressful conditions in marine invertebrates.