The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

作     者：Ruibing Chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng Chen Shouhong Gao Xianghui Chen Qing Li Henan Zhang Wansheng Chen Lei Zhang 

作者机构：Department of Pharmaceutical BotanySchool of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of Chinese Medicine Resources and BiotechnologyShanghai University of Traditional Chinese MedicineShanghai 201203China Department of PharmacyShanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of MedicineShanghai UniversityShanghai 200433China Institute of Edible FungiShanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life Sciences and MedicineKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di HerbsBeijing 100700China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2024年第14卷第1期

页      面：405-420页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 07[理学] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：The authors would like to acknowledge Professor Jiankang Zhu and his lab at the Shanghai Center for Plant Stress Biology,Chinese Center for Plant Stress Biology,for providing the CRISPR/Cas9 system plasmids.All authors declare no competing financial or nonfinancial interests.This work was funded by the National Natural Science Foundation of China(grant Nos.82225047,32000231,31970316,and 32170274) the National Key Research and Development Program of China(grant no.2022YFC3501703) Shanghai Science and Technology Development Funds(23QA1411400,China) Key project at central government level(The ability establishment of sustainable use for valuable Chinese medicine resources,2060302). 

主　　题：Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF 

摘      要：Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human health.Elucidating the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant improvement.Here,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and UGT71B2.DIR1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan content.Mechanistically,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of DIR1.These systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in I.indigotica roots and is also a part of the LTF1-mediated regulatory network to resist stresses.In conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.