Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography

作     者：Wangyan Hu Ke Li Xiangnan Dou Ning Li Xiayan Wang Wangyan Hu;Ke Li;Xiangnan Dou;Ning Li;Xiayan Wang

作者机构：Center Excellence for Environmental Safety and Biological EffectsBeijing Key Laboratory for Green Catalysis and SeparationDepartment of ChemistryBeijing University of TechnologyBeijing 100124China Center for Environmental MetrologyNational Institute of MetrologyBeijing 100029China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第4期

页      面：486-489页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070205[理学-凝聚态物理] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China (No.21936001) the Beijing Outstanding Young Scientist Program (No.BJJWZYJH01201910005017) 

主　　题：Nano-size packing Single-particle frit Packed column Microchip LC Chromatographic separation 

摘      要：Modern chromatography is increasingly focused on miniaturization and integration. Compared to conventional liquid chromatography, microfluidic chip liquid chromatography(microchip-LC) has the potential due to its zero-dead volume connection and ease of integration. Nano-sized packings have the potential to significantly enhance separation performance in microchip-LC. However, their application has been hindered by packing difficulties. This study presents a method for packing nano-sized silica particles into a microchannel as the stationary phase. The microchip-LC packed column was prepared by combining the weir and the porous silica single-particle as frit to retain the packing particles. A surface tensionbased single-particle picking technique was established to insert porous single-particle frit into glass microchannels. Additionally, we developed a slurry packing method that utilizes air pressure to inject nano-sized packing into the microchannel. Pressure-driven chromatographic separation was performed using this nano-packed column integrated into a glass microchip. The mixture of four PAHs was successfully separated within just 8 min using a 5 mm separation channel length, achieving high theoretical plates(10~6plates/m). Overall, these findings demonstrate the potential of utilizing nano-sized packings for enhancing chromatographic performance in microchip systems.