Callus proliferation-induced hypoxic microenvironment decreases shoot regeneration competence in Arabidopsis
作者机构：Department of ChemistrySeoul National UniversitySeoul 08826Korea Plant Genomics and Breeding InstituteSeoul National UniversitySeoul 08826Korea Research Institute of Basic SciencesSeoul National UniversitySeoul 08826Korea
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2024年第17卷第3期
页 面：395-408页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：funded by the Samsung Science and Technology Foundation(South Korea)under Project Number SSTF-BA2001-10 by the New Breeding Technologies Development Program(RS-2024-00322275)of the Rural Development Administration,South Korea
主 题：callus cell proliferation-induced hypoxia SID2 plant regeneration salicylic acid RAP2.12
摘 要：Plants are aerobic organisms that rely on molecular oxygen for respiratory energy *** conditions,with oxygen levels ranging between 1%and 5%,usually limit aerobic respiration and affect plant growth and ***,we demonstrate that the hypoxic microenvironment induced by active cell proliferation during the two-step plant regeneration process intrinsically represses the regener-ation competence of the callus in Arabidopsis *** showed that hypoxia-repressed plant regener-ation is mediated by the RELATED TO APETALA2.12(RAP2.12)protein,a memberof the Ethylene Response Factor VIl(ERF-Vll)*** found that the hypoxia-activated RAP2.12 protein promotes salicylic acid(SA)biosynthesis and defense responses,thereby inhibiting pluripotency acquisition and de novo shoot regeneration in *** and genetic analyses revealed that RAP2.12 could bind directly to the SALICYLIC ACID INDUCTION DEFICIENT 2(SID2)gene promoter and activate SA biosynthesis,repressing plant regeneration possibly via a PLETHORA(PLT)-dependent ***,the rap2.12 mutant calli exhibits enhanced shoot regeneration,which is impaired by SA *** together,these find-ings uncover that the cell proliferation-dependent hypoxic microenvironment reduces cellular pluripotency and plant regeneration through the RAP2.12-SID2 module.
维普期刊数据库