Efficient heterologous biosynthesis of verazine,a metabolic precursor of the anti-cancer drug cyclopamine,in Nicotiana benthamiana
作者机构：College of Life SciencesNorthwest A&F UniversityYangling 712100China Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University)Ministry of EducationHarbin 150040China Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and UtilizationNortheast Forestry UniversityHarbin 150040China Jilin Provincial Key Laboratory of Agricultural BiotechnologyJilin Academy of Agricultural SciencesChangchun 130033China Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese MedicineInstitute of Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China
出 版 物：《Plant Communications》 (植物通讯（英文）)
年 卷 期：2024年第5卷第6期
页 面：1-4页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by the National Key Research and Development Program of China(2023YFA0915800) the Jilin Provincial Agricultural Science&Technology Innovation Project(CXGC202105GH)
主 题：cyclopamine cancer
摘 要：Dear Editor,Among the large number of Veratrum steroid alkaloids(Zhang et al.,2020),cyclopamine has been shown to have various inhibitory mechanisms against cancer,including inhibition of the hedgehog signaling pathway,improvement of tumor tissue microenvironment,inhibition of cell respiration,promotion of cell apoptosis,and reversal of tumor drug resistance(Liu et al.,2023).The biosynthetic pathway of verazine,a possible metabolic precursor of cyclopamine,has been discovered in Veratrum californicum(Augustin et al.,2015).However,the content of Veratrum steroid alkaloids in plants is very low(Supplemental Figure 2),so reliance on direct extraction from plants for commercial production is not feasible.A more effective strategy is to synthesize rare plant secondary metabolites using synthetic biology technology(Liu et al.,2022).
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