Characteristics of N^(6)-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii

作     者：Ying Lv Fei Han Mengxia Liu Ting Zhang Guanshen Cui Jiaojiao Wang Ying Yang Yun-Gui Yang Wenqiang Yang 

作者机构：Photosynthesis Research CenterKey Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical GardenBeijing 100093China College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China CAS Key Laboratory of Genomic and Precision MedicineBeijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China Sino-Danish CollegeUniversity of Chinese Academy of SciencesBeijing 100049China Institute of Stem Cell and RegenerationChinese Academy of SciencesBeijing 100101China Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100093China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2023年第21卷第4期

页      面：756-768页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Key R&D Program of China(Grant Nos.2019YFA0904600,2018YFA0801200,and 2021YFA0910800) the National Natural Science Foundation of China(Grant Nos.31870217 and 91940304). 

主　　题：N6-methyladenosine m^(6)A sequencing Sexual reproduction Microtubule-associated pathway Photosynthesis Chlamydomonas reinhardtii 

摘      要：The unicellular green alga Chlamydomonas reinhardtii(hereafter Chlamydomonas)possesses both plant and animal attributes,and it is an ideal model organism for studying fundamental processes such as photosynthesis,sexual reproduction,and life cycle.N^(6)-methyladenosine(m^(6)A)is the most prevalent mRNA modification,and it plays important roles during sexual reproduction in animals and plants.However,the pattern and function of m^(6)A modification during the sexual reproduction of Chlamydomonas remain unknown.Here,we performed transcriptome and methylated RNA immunoprecipitation sequencing(MeRIP-seq)analyses on six samples from different stages during sexual reproduction of the Chlamydomonas life cycle.The results show that m^(6)A modification frequently occurs at the main motif of DRAC(D=G/A/U,R=A/G)in Chlamydomonas mRNAs.Moreover,m^(6)A peaks in Chlamydomonas mRNAs are mainly enriched in the 30 untranslated regions(30 UTRs)and negatively correlated with the abundance of transcripts at each stage.In particular,there is a significant negative correlation between the expression levels and the m^(6)A levels of genes involved in the microtubule-associated pathway,indicating that m^(6)A modification influences the sexual reproduction and the life cycle of Chlamydomonas by regulating microtubule-based movement.In summary,our findings are the first to demonstrate the distribution and the functions of m^(6)A modification in Chlamydomonas mRNAs and provide new evolutionary insights into m^(6)A modification in the process of sexual reproduction in other plant organisms.