Engineering humanized mice for improved hematopoietic reconstitution
Engineering humanized mice for improved hematopoietic reconstitution作者机构：Koch Institute for Integrative Cancer Research and Department of Biology Massachusetts Institute of Technology Cambridge MA USA Interdisciplinary Research Group in Infectious Diseases Singapore-MIT AlLiance for Research and Technology Singapore
出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)
年 卷 期：2012年第9卷第3期
页 面：215-224页
核心收录：
学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 09[农学] 071009[理学-细胞生物学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：Supported by funds from the Singapore-MIT Alliance for Research and Technology Ragon Institute of MGH, MIT and Harvard (to JC)
主 题：cytokines expansion genetic modification hematopoietic stem cells humanized mice
摘 要：Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.
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