A simple and efficient in planta transformation method based on the active regeneration capacity of plants

作     者：Guoguo Mei Ao Chen Yaru Wang Shuquan Li Minyi Wu Yilong Hu Xu Liu Xingliang Hou 

作者机构：Guangdong Provincial Key Laboratory of Applied Botany&State Key Laboratory of Plant Diversity and Specialty CropsSouth China Botanical GardenChinese Academy of SciencesGuangzhou 510650China University of Chinese Academy of SciencesBeijing 100049China These authors contributed equally to this article. 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2024年第5卷第4期

页      面：166-176页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by grants from the Key Area Research and Development Program of Guangdong Province (2022B1111230001) the Science and Technology Projects in Guangzhou (E3330900-01) the National Natural Science Foundation of China-Guangdong Joint Fund (U1701234) the Guangdong Special Support Plan Project (2019TQ05N140) the Guangzhou Municipal Science and Technology Project (202201010641) the Guangdong Forestry Bureau (Key Laboratory of Plant Ex Situ Protection and Utilization in South China) (E336030011) 

主　　题：plant genetic transformation RAPID active regeneration sweet potato potato bayhops 

摘      要：Plant genetic transformation strategies serve as essential tools for the genetic engineering and advanced molecular breeding of ***,the complicated operational protocols and low efficiency of cur-rent transformation strategies restrict the genetic modification of most plant *** paper de-scribes the development of the regenerative activity–dependent in planta injection delivery(RAPID)method based on the active regeneration capacity of *** this method,Agrobacterium tumefaciens is delivered to plant meristems via injection to induce transfected nascent *** transgenic plants can be obtained by subsequent vegetative propagation of the positive nascent *** method was successfully used for transformation of plants with strong regeneration capacity,including different genotypes of sweet potato(Ipomoea batatas),potato(Solanum tuberosum),and bayhops(Ipo-moea pes-caprae).Compared with traditional transformation methods,RAPID has a much higher trans-formation efficiency and shorter duration,and it does not require tissue culture *** RAPID method therefore overcomes the limitations of traditional methods to enable rapid in planta transformation and can be potentially applied to a wide range of plant species that are capable of active regeneration.