Screening the Synergistic Components of Acetylcholinesterase Inhibition from Phellodendron Bark Based on Fingerprint-Activity Relationship Modeling

作     者：Hong-Shan Zhou Qi-Ming Luo Xiao-Bing Lin Chuan-Sheng Zhu Ping Li Wen Gao Hong-Shan Zhou;Qi-Ming Luo;Xiao-Bing Lin;Chuan-Sheng Zhu;Ping Li;Wen Gao

作者机构：State Key Laboratory of Natural MedicinesSchool of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjingChina 

出 版 物：《World Journal of Traditional Chinese Medicine》 (世界中医药杂志（英文）)

年 卷 期：2023年第9卷第4期

页      面：461-468页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Key R&D Program of China(Nos.2018YFC1707900 and 2019YFC1711000) 

主　　题：Berberine combination index high-performance chromatography fingerprint jatrorrhizine palmatine 

摘      要：Objective:Phellodendron chinense(PC)bark and Phellodendron amurense(PA)bark are two herbal medicines recorded in the Chinese Pharmacopoeia(2020 edition)that are easily mistaken for one *** this study,the chemical constituents of PC and PA were compared using chromatographic fingerprints,and the potential synergistic acetylcholinesterase(ACh E)inhibitor components were screened based on the correlation of fingerprint *** and Methods:Chromatographic fingerprints based on high-performance liquid chromatography were developed for the analysis and comparison of chemical compounds in PC and PA *** ACh E inhibitory activity of PC and PA was determined using the Ellman ***,the contribution of the characterized alkaloids in PC and PA to the overall ACh E inhibition was modeled using partial least squares regression(PLSR).Results:The total alkaloid content in PC was higher than that in PA,which causes PC to have stronger anti-ACh E ***,13 and 20 common peaks were identified in the PC and PA samples,*** them,berberine(BER)was the dominant alkaloid in PC,which covered more than 65%of the total peak area in PC,but only approximately 25%of that in PA,indicating that the chemical composition is different between PC and *** spectrum–effect analysis based on PLSR and the correlation analysis showed that the BER-palmatine(PAL)and BER-jatrorrhizine(JAT)pairs have a synergistic inhibitory effect on ACh E ***:A high-performance chromatographic fingerprint was established to distinguish PC and *** efficacy-associated markers were screened,including the pairs of BER-PAL and BER-JAT with anti-ACh E activity,and the findings may assist with the quality control of PA and PC.