Electroacupuncture Promotes Functional Recovery after Facial Nerve Injury in Rats by Regulating Autophagy via GDNF and PI3K/m TOR Signaling Pathway

作     者：YAO Jun-peng FENG Xiu-mei WANG Lu LI Yan-qiu ZHU Zi-yue YAN Xiang-yun YANG Yu-qing LI Ying ZHANG Wei YAO Jun-peng;FENG Xiu-mei;WANG Lu;LI Yan-qiu;ZHU Zi-yue;YAN Xiang-yun;YANG Yu-qing;LI Ying;ZHANG Wei

作者机构：Acupuncture and Tuina SchoolChengdu University of Traditional Chinese MedicineChengdu611137China Department of Rehabilitation MedicineGuanghan People’s HospitalGuanghanSichuan Province618399China Academic Affairs OfficeChengdu University of Traditional Chinese MedicineChengdu611137China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2024年第30卷第3期

页      面：251-259页

核心收录：

学科分类：1005[医学-中医学] 1002[医学-临床医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China (No.81603706) 

主　　题：electroacupuncture facial nerve injury autophagy glial cell line-derived neurotrophic factor phosphatidylinositol-3-kinase/mammalian target of rapamycin Chinese medicine 

摘      要：Objective:To explore the mechanism of electroacupuncture(EA) in promoting recovery of the facial function with the involvement of autophagy,glial cell line-derived neurotrophic factor(GDNF),and phosphatidylinositol-3-kinase(PI3K)/mammalian target of rapamycin(mTOR) signaling ***:Seventy-two male Sprague-Dawley rats were randomly allocated into the control,sham-operated,facial nerve injury(FNI),EA,EA+3-methyladenine(3-MA),and EA+GDNF antagonist groups using a random number table,with 12 rats in each *** FNI rat model was established with facial nerve crushing *** intervention was conducted at Dicang(ST 4),Jiache(ST 6),Yifeng(SJ 17),and Hegu(LI 4) acupoints for 2 *** Simone’s 10-Point Scale was utilized to monitor the recovery of facial *** histopathological evaluation of facial nerves was performed using hematoxylin-eosin(HE) *** levels of Beclin-1,light chain 3(LC3),and P62 were detected by immunohistochemistry(IHC),immunofluorescence,and reverse transcriptionpolymerase chain reaction,***,IHC was also used to detect the levels of GDNF,Rai,PI3K,and ***:The facial functional scores were significantly increased in the EA group than the FNI group(P0.05 or P0.01).HE staining showed nerve axons and myelin sheaths,which were destroyed immediately after the injury,were recovered with EA *** expressions of Beclin-1 and LC3 were significantly elevated and the expression of P62 was markedly reduced in FNI rats(P0.01);however,EA treatment reversed these abnormal changes(P0.01).Meanwhile,EA stimulation significantly increased the levels of GDNF,Rai,PI3K,and mTOR(P0.01).After exogenous administration with autophagy inhibitor 3-MA or GDNF antagonist,the repair effect of EA on facial function was attenuated(P0.05 or P0.01).Conclusions:EA could promote the recovery of facial function and repair the facial nerve damages in a rat model of *** may exert this neuroreparative effect through