MB49小鼠膀胱癌腺病毒基因疗法模型的优化
Optimization of the MB49 mouse bladder cancer model for adenoviral gene therapy作者机构：Clinical Immunology Division Rudbeck Laboratory Uppsala University Sweden Division of Molecular Medicine Uppsala University Hospital Sweden Experimental Urology Division Rudbeck LaboratoryUppsala University Sweden 不详
出 版 物：《中国比较医学杂志》 (Chinese Journal of Comparative Medicine)
年 卷 期：2006年第16卷第3期
页 面：166-166页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：MB49 小鼠 膀胱癌 腺病毒 基因疗法 模型 优化
摘 要：Bladder cancer is regarded as a promising candidate for innovative therapies in the field of immune and gene therapy. In this paper, we present the subcutaneous, metastatic and a novel orthotopic model of murine MB49 bladder cancer in C57BL/6 mice. We further show the potential of using adenoviral vectors together with different transduction enhancers to augment in vivo gene delivery. Finally, we present candidate genes for tumour detection, therapy or targeting. The MB49 tumour grew rapidly in mice. The subcutaneous model allowed for tumour detection within a week and the possibility to monitor growth rate on a day-by-day basis. Injection of MB49 cells intravenously into the tail vein gave rise to lung metastases within 16 days, while instillation of tumour ceils into pretreated bladders led to a survival time of 20－40 days.
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