Insight into the spoilage heterogeneity of meat-borne bacteria isolates with high-producing collagenase

作     者：Haodong Wang Liangting Shao Jinhao Zhang Xinglian Xu Jianjun Li Huhu Wang Haodong Wang;Liangting Shao;Jinhao Zhang;Xinglian Xu;Jianjun Li;Huhu Wang

作者机构：Jiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety ControlCollege of Food Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Yantai XiWang Meat Food Co.Ltd.Yantai 264000China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2024年第13卷第3期

页      面：1402-1409页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程] 

基　　金：financed by grants from the Natural Science Foundation of Jiangsu Province in China (BK20221515) the National Natural Science Foundation of China (32172266) 

主　　题：Bacteria Chilled chicken Heterogeneity Collagenase Spoilage 

摘      要：Chilled chicken is inevitably contaminated by microorganisms during slaughtering and processing,resulting in *** parts of chilled chicken,especially wings,feet,and other skin-on products,are abundant in collagen,which may be the primary target for degradation by spoilage *** this work,a total of 17 isolates of spoilage bacteria that could secrete both collagenase and lipase were determined by raw-chicken juice agar(RJA)method,and the results showed that 7 strains of Serratia,Aeromonas,and Pseudomonas could significantly decompose the collagen *** gelatin zymography showed that Serratia liquefaciens(F5)and(G7)had apparent degradation bands around 50 kDa,and Aeromonas veronii(G8)and Aeromonas salmonicida(H8)had a band around.65 and 95 kDa,*** lipase and collagenase activities were detected isolate-by-isolate,with F5 showing the highest collagenase *** spoilage ability on meat in situ,F5 performed strongest in spoilage ability,indicated by the total viable counts,total volatile basic nitrogen content,sensory scores,lipase,and collagenase *** study provides a theoretical basis for spoilage heterogeneity of strains with high-producing collagenase in meat.