Evaluation of combined detection of nuclear factor erythroid 2-related factor 2 and glutathione peroxidase 4 in primary hepatic carcinoma and preliminary exploration of pathogenesis
作者机构：Department of Hepatobiliary SurgeryThe Second Hospital of NanjingNanjing Hospital Affiliated to Nanjing University of Traditional Chinese MedicineNanjing210003China
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2023年第47卷第12期
页 面：2609-2615页
核心收录：
学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Nuclear factor erythroid 2 Related factor 2 Glutathione peroxidase 4 Primary hepatic carcinoma Clinical significance Mechanism of action Pathogenesis
摘 要：This study aims to analyze the clinical significance and mechanism of nuclear factor erythroid 2-related factor 2(NRF2)and glutathione peroxidase 4(GPX4)in primary hepatic carcinoma(PHC).Methods:The expression of NRF2 and GPX4 in peripheral blood of patients with PHC was determined to analyze the diagnostic value of the two combined for *** prognostic significance of NRF2 and GPX4 was evaluated by 3-year *** liver epithelial cells THLE-2 and human hepatocellular carcinoma cells HepG2 were purchased,and the expression of NRF2 and GPX4 in the cells was ***2 and GPX4 aberrant expression vectors were constructed and transfected into HepG2,and changes in cell proliferation and invasion capabilities were ***:The expression of NRF2 and GPX4 in patients with PHC was higher than that in patients with LC or VH(p0.05),and the two indicators combined was excellent in diagnosing ***,patients with high expression of NRF2 and GPX4 had a higher risk of death(p0.05).In in vitro experiments,both NRF2 and GPX4 expression was elevated in HepG2(p0.05).HepG2 activity was enhanced by increasing the expression of the two,vice versa(p0.05).Conclusion:NRF2 and GPX4 combined is excellent in diagnosing PHC,and promotes the malignant development of PHC.
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