Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

作     者：ZHONG XingYu TIAN YuQing NIU GuoQing TAN HuaRong 

作者机构：State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences University of Chinese Academy of Sciences 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2013年第56卷第7期

页      面：609-618页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by grants from the Ministry of Science and Technology of China (2013CB734001) the National Natural Science Foundation of China (31270110, 31030003) the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-J-6) 

主　　题：secondary metabolite gene cluster assemble features draft genome 

摘      要：A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters.