Suspended particulate matter promotes epithelial-to-mesenchymal transition in alveolar epithelial cells via TGF-β1-mediated ROS/IL-8/SMAD3 axis

作     者：Shahrzad Molavinia Dian Dayer Mohammad Javad Khodayar Gholamreza Goudarzi Maryam Salehcheh Shahrzad Molavinia;Dian Dayer;Mohammad Javad Khodayar;Gholamreza Goudarzi;Maryam Salehcheh

作者机构：Cellular and Molecular Research CenterMedical Basic Sciences Research InstituteAhvaz Jundishapur University of Medical SciencesAhvazIran Department of ToxicologySchool of PharmacyAhvaz Jundishapur University of Medical SciencesAhvazIran Student Research CommitteeAhvaz Jundishapur University of Medical SciencesAhvazIran Toxicology Research CenterMedical Basic Sciences Research InstituteAhvaz Jundishapur University of Medical SciencesAhvazIran Air Pollution and Respiratory Diseases Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran Iranian Scientific Association of Clean AirTehranIran 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2024年第141卷第7期

页      面：139-150页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 07[理学] 070602[理学-大气物理学与大气环境] 0706[理学-大气科学] 100402[医学-劳动卫生与环境卫生学] 10[医学] 

基　　金：the financial support(Grant Number:CMRC9923)was provided by the Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran 

主　　题：Particulate matter TGF-β1 Oxidative stress Epithelial-mesenchymal transition Pulmonary fibrosis Cells 

摘      要：Epidemiological evidence presents that dust storms are related to respiratory diseases,such as pulmonary fibrosis(PF).However,the precise underlying mechanisms of SPM-elicited adverse effects still need to be ***-mesenchymal transition(EMT)process is a characteristic of *** discussed whether suspended particulate matter(SPM)is involved in EMT induction via transforming growth factor-β1(TGF-β1).In this study,a detailed elemental analysis(55 elements),particle size,and morphology were *** investigate the toxicity of SPM,an MTT test was performed to detect cell ***,A549cells were exposed to selected concentrations of SPM(20 and 40μg/mL)for single and repeated *** DCFH-DA assay showed that exposure to SPM could produce reactive oxygen species(ROS).The ELISA assay demonstrated increased levels of interleukin-8(IL-8)and TGF-β1 in the *** blot was used to detect the expression of proteins associated with EMT and the SMAD3-dependent *** of western blot demonstrated that E-cadherin was reduced,whereas p-SMAD3,vimentin,andα-smooth muscle actin were *** findings indicated that SPM triggered EMT by induction of oxidative stress,inflammation,and the TGF-β1/SMAD3 pathway activation.