Dynamic Measurement of Intracellular pH Based on Bioluminescent Bacteria
作者机构：State Key Laboratory of Analytical Chemistry for Life ScienceSchool of Chemistry and Chemical EngineeringChemBIC(Chemistry and Biomedicine Innovation Center)Nanjing UniversityNanjing210023P.R.China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2024年第40卷第2期
页 面：287-292页
核心收录：
学科分类：07[理学] 0703[理学-化学] 0701[理学-数学] 070101[理学-基础数学]
基 金：supported by the National Natural Science Foundation of China(Nos.21925403,21874070) the Excellent Research Program of Nanjing University,China(No.ZYJH004)
主 题：Bioluminescence P.phosphoreum Intracellular pH Cell imaging
摘 要：Intracellular pH(pHi)is a fundamental indicator of cellular physiological state,regulating cellular state and function,and has important research *** various probes for measuring intracellular pH were available,it is challenging to reflect pHi in real-time and reversible ***,we developed a whole-cell bioluminescent(BL)probe based on wild type BL bacteria,photobacterium phosphoreum(***),to determine and image *** dependence of BL intensity of *** on pH values of culture solutions was *** was found that BL intensity could respond to the change of pH values rapidly and *** further revealed that *** maintained pH homeostasis in the extracellular pH(pHe)within the range of 5.0–7.0,while intracellular pH homeostasis was destroyed at the alkaline *** method opens up the enormous potential of BL bacteria as an alternative to fluorescence for monitoring and imaging pHi.
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