CsABF3-activated CsSUT1 pathway is implicated in pre-harvest water deficit inducing sucrose accumulation in citrus fruit

作     者：Xiaochuan Ma Yuanyuan Chang Feifei Li Junfeng Yang Li Ye Tie Zhou Yan Jin Ling Sheng Xiaopeng Lu Xiaochuan Ma;Yuanyuan Chang;Feifei Li;Junfeng Yang;Li Ye;Tie Zhou;Yan Jin;Ling Sheng;Xiaopeng Lu

作者机构：College of HorticultureHunan Agricultural UniversityChangshaHunan 410128China National Center for Citrus ImprovementChangshaHunan 410128China Institute of HorticultureHunan Academy of Agricultural ScienceChangshaHunan 410125China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2024年第10卷第1期

页      面：103-114页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.32172520) the earmarked fund for China Agriculture Research System(Grant No.CARS-26) 

主　　题：Citrus Water deficit Pre-harvest Sucrose CsABF3 CsSUT1 

摘      要：Pre-harvest water deficit(PHWD)plays an important role in sugar accumulation of citrus ***,the mechanism is not known ***,it was confirmed that PHWD promoted sucrose accumulation of citrus fruit,but had limited effect on fructose,glucose and total acid.A sucrose transporter,Cs SUT1,which localizes to the plasma membrane,was demonstrated to function in sucrose transport induced by *** to wild-type,Cs SUT1 overexpression in citrus calli stimulated sucrose,fructose and glucose accumulation,while its silencing in juice sacs reduced sucrose *** sugar accumulation in transgenic lines enhanced plant drought tolerance,and resulted in decreased electrolyte leakage,malondialdehyde and hydrogen peroxide contents,as well as increased superoxide dismutase activity and proline *** abscisic acid(ABA)-responsive transcription factor,Cs ABF3,was found with a same expression pattern with Cs SUT1 under *** one-hybrid,electrophoretic mobility shift assay and dual-luciferase assays all revealed that Cs ABF3 directly bound with the Cs SUT1 promoter by ABA responsive *** Cs ABF3 was overexpressed in citrus calli,the sucrose,fructose and glucose concentration increased ***,transgenic studies demonstrated that Cs ABF3 could affect sucrose accumulation by regulating Cs ***,this study revealed a regulation of Cs ABF3 promoting Cs SUT1 expression and sucrose accumulation in response to *** results provide a detail insight into the quality formation of citrus fruit.