Construction of Plant Expression Vector for Hand,Foot and Mouth Virus EV71-VP1 Gene and Its Expression in Tomato

作     者：Wang Mei-liang Zhao Yue Wang Yu-dan Li Xin-zhi Zhang Yao Chen Xiu-ling Qiu You-wen Wang Ao-xue Wang Mei-liang;Zhao Yue;Wang Yu-dan;Li Xin-zhi;Zhang Yao;Chen Xiu-ling;Qiu You-wen;Wang Ao-xue

作者机构：College of Life SciencesNortheast Agricultural UniversityHarbin 150030China College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2023年第30卷第4期

页      面：53-62页

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the Natural Science Foundation of Heilongjiang Province(LH2021C032) 

主　　题：hand foot and mouth disease(HFMD) EV71-VP1 tomato plant transgenic vaccine vector construction 

摘      要：EV71-type virus is one of the main pathogens causing the occurrence of hand,foot and mouth disease(HFMD),and VP1 protein,a factor that directly determines the antigenicity of the virus,has been *** tomato was selected as a bioreactor for the production of an edible EV71 vaccine designed for the VP1 capsid *** molecular biology techniques,the fusion gene EV71-VP1 was cut from vector PGEX-4T-2,a vector containing the p2300-EV71 gene with CaMV35S promoter and TL regulatory elements was constructed,and the hypocotyl and cotyledons of tomato were transformed using Agrobacterium(EHA105)-mediated method,screened,elongated and rooted,and finally 20 resistant tomato plants were *** transgenic positive seedlings were obtained by digestion and PCR assay,among which three plants were detected by RT-PCR to be capable of transcriptional translation at the RNA *** experimental results aimed to explore new material support for the preparation of transgenic plant oral vaccines against EV71 infection and provide a theoretical basis for accelerating the development of transgenic plant vaccines in the future.