Perturbation of autophagy pathways in murine alveolar macrophage by 2D TMDCs is chalcogen-dependent

作     者：Xiaofei Zhou Weitao Jin Rui Zhang Xuan Mao Jianbo Jia Hongyu Zhou 

作者机构：College of Science&TechnologyHebei Agricultural UniversityHuanghua 061100China Institute of Environmental Research at Greater Bay AreaKey Laboratory for Water Quality and Conservation of the Pearl River DeltaMinistry of EducationGuangzhou UniversityGuangzhou 510006China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2024年第135卷第1期

页      面：97-107页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

基　　金：supported by the Special Scientific Research Fund for Talents Introduced of Hebei Agricultural University (No.YJ2019030) the National Natural Science Foundation of China (Nos.22276042,21906035) the Pearl River Young Talents Program of Guangdong Province (No.2017GC010269)。 

主　　题：Chalcogen group Autophagy pathway Cell function Endosomal escape Inhalation toxicity 

摘      要：Increasing risks of incidental and occupational exposures to two-dimensional transition metal dichalcogenides(2D TMDCs)due to their broad application in various areas raised their public health concerns.While the composition-dependent cytotoxicity of 2D TMDCs has been well-recognized,how the outer chalcogenide atoms and inner transition metal atoms differentially contribute to their perturbation on cell homeostasis at non-lethal doses remains to be identified.In the present work,we compared the autophagy induction and related mechanisms in response to WS_(2),NbS_(2),WSe_(2)and Nb Se_(2)nanosheets exposures in MH-S murine alveolar macrophages.All these 2D TMDCs had comparable physicochemical properties,overall cytotoxicity and capability in triggering autophagy in MH-S cells,but showed outer chalcogen-dependent subcellular localization and activation of autophagy pathways.Specifically,WS_(2)and NbS_(2)nanosheets adhered on the cell surface and internalized in the lysosomes,and triggered m TOR-dependent activation of autophagy.Meanwhile,WSe_(2)and Nb Se_(2)nanosheets had extensive distribution in cytoplasm of MH-S cells and induced autophagy in an m TOR-independent manner.Furthermore,the 2D TMDCs-induced perturbation on autophagy aggravated the cytotoxicity of respirable benzo[a]pyrene.These findings provide a deeper insight into the potential health risk of environmental 2D TMDCs from the perspective of homeostasis perturbation.