The direct and gut microbiota-mediated effects of dietary bile acids on the improvement of gut barriers in largemouth bass(Micropterus salmoides)

作     者：Rui Xia Qingshuang Zhang Dongmei Xia Qiang Hao Qianwen Ding Chao Ran Yalin Yang Aizhi Cao Zhen Zhang Zhigang Zhou Rui Xia;Qingshuang Zhang;Dongmei Xia;Qiang Hao;Qianwen Ding;Chao Ran;Yalin Yang;Aizhi Cao;Zhen Zhang;Zhigang Zhou

作者机构：China-Norway Joint Lab on Fish Gut MicrobiotaInstitute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway-China Joint Lab on Fish Gut MicrobiotaInstitute of BiologyNorwegian University of Science and TechnologyTrondheimNorway Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural AffairsInstitute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Shandong Longchang Animal Health Care Co.Ltd.Jinan 251100China Institute of Animal Husbandry and VeterinaryJiangxi Academy of Agricultural ScienceJiangxi 330000China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第14卷第3期

页      面：32-42页

核心收录：

学科分类：1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 10[医学] 

基　　金：funded by National Natural Science Foundation of China(NSFC 32061133004 and 31925038)。 

主　　题：Bile acids Gut barrier Gut microbiota Largemouth bass Germ-free zebrafish 

摘      要：Fish gut barrier damage under intensive culture model is a significant concern for aquaculture industry.This study aimed to investigate the effects of bile acids(BAs)on gut barriers in Micropterus salmoides.A germ-free(GF)zebrafish model was employed to elucidate the effects of the direct stimulation of BAs and the indirect regulations mediated by the gut microbiota on gut barrier functions.Four diets were formulated with BAs supplemented at 0,150,300 and 450 mg/kg,and these 4 diets were defined as control,BA150,BA300 and BA450,respectively.After 5 weeks of feeding experiment,the survival rate of fish fed with BA300 diet was increased(P0.05).The results of the gut microbiota transferring experiment demonstrated an upregulation of gut barrier-related genes,including immunoglobulin Z/T(Ig Z/T),IL-6,IL-1βand IL-10,by the gut microbiota transferred from the BA300 group compared with the control(P0.05).Feeding the BA300 diet directly to GF zebrafish resulted in enhanced expression of Ig M,Ig Z/T,lysozyme,occludin-2,IL-6 and IL-10(P0.05).In conclusion,BAs can improve the gut barriers of fish through both direct and indirect effects mediated by the gut microbiota.