A micro-fragmented collagen gel as a cell-assembling platform for critical limb ischemia repair
作者机构：Center for BiomaterialsBiomedical Research InstituteKorea Institute of Science and Technology(KIST)Seoul02792Republic of Korea Division of Bio-Medical Science and TechnologyKIST SchoolUniversity of Science and TechnologySeoul02792Republic of Korea Department of Applied BioengineeringGraduate School of Convergence Science and TechnologySeoul National UniversitySeoul08826Republic of Korea Research Institute for Convergence ScienceSeoul National UniversitySeoul08826Republic of Korea Department of Genetics and BiotechnologyCollege of Life SciencesKyung Hee UniversityYongin-siGyeonggi-do17104Republic of Korea S.Biomedics Co.Ltd.Seoul04797Republic of Korea
出 版 物：《Bioactive Materials》 (生物活性材料（英文）)
年 卷 期：2024年第34卷第4期
页 面：80-97页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by a grant from the Korean Fund for Regenerative Medicine funded by the Ministry of Science and ICT and the Ministry of Health&Welfare of Korea[22C0620L1-11]
主 题：Regenerative medicine Stem cell therapy Collagen microgel 3D cell culture Critical limb ischemia
摘 要：Critical limb ischemia(CLI)is a devastating disease characterized by the progressive blockage of blood *** the paracrine effect of growth factors in stem cell therapy made it a promising angiogenic therapy for CLI,poor cell survival in the harsh ischemic microenvironment limited its ***,an imperative need exists for a stem-cell delivery method that enhances cell ***,a collagen microgel(CMG)cell-delivery scaffold(40×20μm)was fabricated via micro-fragmentation from collagen-hyaluronic acid polyionic complex to improve transplantation *** human adipose-derived stem cells(hASCs)with CMG enabled integrin receptors to interact with CMG to form injectable 3-dimensional constructs(CMG-hASCs)with a microporous microarchitecture and enhanced mass ***-hASCs exhibited higher cell survival(p0.0001)and angiogenic potential in tube formation and aortic ring angiogenesis assays than cell *** of CMG-hASCs intramuscularly into CLI mice increased blood perfusion and limb salvage ratios by 40%and 60%,respectively,compared to cell aggregate-treated *** immunofluorescent analysis revealed that transplanted CMG-hASCs have greater muscle regenerative and angiogenic potential,with enhanced cell survival than cell aggregates(p0.05).Collectively,we propose CMG as a cell-assembling platform and CMG-hASCs as promising therapeutics to treat CLI.
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