Exposure to Electromagnetic Fields from Mobile Phones and Fructose consumption Coalesce to Perturb Metabolic Regulators AMPK/SIRT1-UCP2/FOXO1 in Growing Rats

作     者：Ruchi Tripathi Sanjay Kumar Banerjee Jay Prakash Nirala Rajani Mathur Ruchi Tripathi;Sanjay Kumar Banerjee;Jay Prakash Nirala;Rajani Mathur

作者机构：Department of PharmacologyDelhi Institute of Pharmaceutical Sciences & ResearchDelhi Pharmaceutical Sciences and Research University(DPSR-U))New DelhiINDIA Drug Discovery Research CentreTranslational Health Science and Technology InstituteFaridabadINDIA Department of BiotechnologyNational Institute of Pharmaceutical Education and ResearchGuwahatiINDIA School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiINDIA 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2023年第36卷第11期

页      面：1045-1058页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the Indian Council of Medical Research(ICMR)in New Delhi.(Sanction NO:5/10/FR/13/2015-RBMH) 

主　　题：EMF-mobile phone Fructose Childhood-adolescence Insulin receptor signal Mitochondrial OxPHOS Antioxidant system Hypothalamic insulin resistance Hepatic insulin resistance 

摘      要：Objective In this study,the combined effect of two stressors,namely,electromagnetic fields(EMFs)from mobile phones and fructose consumption,on hypothalamic and hepatic master metabolic regulators of the AMPK/SIRT1-UCP2/FOXO1 pathway were elucidated to delineate the underlying molecular mechanisms of insulin *** Weaned Wistar rats(28 days old)were divided into 4 groups:Normal,Exposure Only(ExpO),Fructose Only(FruO),and Exposure and Fructose(EF).Each group was provided standard laboratory chow ad libitum for 8 ***,the control groups,namely,the Normal and FruO groups,had unrestricted access to drinking water and fructose solution(15%),***,the respective treatment groups,namely,the ExpO and EF groups,received EMF exposure(1,760 MHz,2 h/day x 8 weeks).In early adulthood,mitochondrial function,insulin receptor signaling,and oxidative stress signals in hypothalamic and hepatic tissues were assessed using western blotting and biochemical *** In the hypothalamic tissue of EF,SIRT1,FOXO 1,p-PI3K,p-AKT,ComplexⅢ,UCP2,MnSOD,and catalase expressions and OXPHOS and GSH activities were significantly decreased(P0.05)compared to the Normal,ExpO,and FruO *** hepatic tissue of EF,the p-AMPKα,SIRT1,FOXO1,IRS1,p-PI3K,ComplexⅠ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,UCP2,and MnSOD expressions and the activity of OXPHOS,SOD,catalase,and GSH were significantly reduced compared to the Normal group(P0.05).Conclusion The findings suggest that the combination of EMF exposure and fructose consumption during childhood and adolescence in Wistar rats disrupts the closely interlinked and multi-regulated crosstalk of insulin receptor signals,mitochondrial OXPHOS,and the antioxidant defense system in the hypothalamus and liver.