STING and TLR9 agonists synergistically enhance the immunogenicity of SARS-CoV-2 subunit vaccine
作者机构：CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China University of Chinese Academy of SciencesBeijing 100049China The GBA National Institute for Nanotechnology InnovationGuangzhou 510700China Guangdong Provincial Institute of Public HealthGuangdong Provincial Center for Disease Control and PreventionGuangzhou 511430China Research Unit of Nanoscience and TechnologyChinese Academy of Medical SciencesBeijing 100049China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第12期
页 面：13322-13334页
核心收录：
学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100102[医学-免疫学] 10[医学]
基 金：supported by the National Basic Research Program of China(Nos.2022YFA1603701 and 2021YFA1200900) Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000) the National Natural Science Foundation of China(Nos.82341044 and 22027810) CAMS Innovation Fund for Medical Sciences(No.CIFMS 2019-I2M-5-018)
主 题：dual-adjuvant system synergistic immune activation antigen dosage reducing variants neutralizing
摘 要：Vaccines that are reliable and efficacious are essential in the fight against the COVID-19 *** this study,we designed a dual-adjuvant system with two pathogen-associated molecular patterns(PAMPs),MnOx and *** system can improve the retention of antigens at the injection site,facilitate pro-inflammatory cytokines secretion,further recruit and activate dendritic cells(DCs).As a result,antigens can be delivered to lymph nodes specifically,and adaptive immunity was *** immunized group showed an enhanced and broadened humoral and cellular immune response in systemic immunity and lung protection when combined with a tandem repeat-linked dimeric antigen version of the SARS-CoV-2 receptor binding domain(RBDdimer).Remarkably,even with a significant reduction in antigen dosage(three times lower)and a decrease in injection frequencies,our nanovaccine was able to produce the highest neutralizing antibody titers against various *** titers were four-fold higher for the wild-type strain and two-fold higher for both the Beta and Omicron variants in comparison with those elicited by the Alum adjuvant *** conclusion,our dual-adjuvant formulation presents a promising protein subunit-based candidate vaccine against SARS-CoV-2.
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