The transition from 2G to 3G-feedstocks enabled efficient production of fuelsand chemicals

作     者：Kai Wang Changsheng Su Haoran Bi Changwei Zhang Di Cai Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan Kai Wang;Changsheng Su;Haoran Bi;Changwei Zhang;Di Cai;Yanhui Liu;Meng Wang;Biqiang Chen;Jens Nielsen;Zihe Liu;Tianwei Tan

作者机构：College of Life Science and TechnologyBeijing University of Chemical TechnologyBeijing Advanced Innovation Center for Soft Matter Science and EngineeringNo.15 North 3rd Ring Rd EastBeijing100029China National Energy R&D Center for BiorefneryBeijing Key Lab of BioprocessBeijing University of Chemical TechnologyNo.15 North 3rd Ring Rd EastBeijing100029China Department of Life SciencesChalmers University of TechnologyGothenburgSE41296Sweden 

出 版 物：《Green Energy & Environment》 (绿色能源与环境（英文版）)

年 卷 期：2024年第9卷第11期

页      面：1759-1770页

核心收录：

学科分类：081702[工学-化学工艺] 0817[工学-化学工程与技术] 08[工学] 

基　　金：supported by the National Key R&D Program of China[2021YFC2103500] National Natural Science Foundation of China(22211530047) Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project[grant numbers:TSBICIP-KJGG-009] the Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology 

主　　题：transition utilized Benson 

摘      要：For decades micoorganisms have been engineered for the utilization of lignocellulose-based second-generation (2G) feedstocks, but with theconcerns of increased levels of atmospheric CO_(2) causing global warming there is an emergent need to transition from the utilization of 2Gfeedstocks to third-generation (3G) feedstocks such as CO_(2) and its derivatives. Here, we established a yeast platform that is capable ofsimultaneously converting 2G and 3G feedstocks into bulk and value-added chemicals. We demonstrated that by adopting 3G substrates such asCO_(2) and formate, the conversion of 2G feedstocks could be substantially improved. Specifically, formate could provide reducing power andenergy for xylose conversion into valuable chemicals. Simultaneously, it can form a concentrated CO_(2) pool inside the cell, providing thermodynamically and kinetically favoured amounts of precursors for CO_(2) fixation pathways, e.g., the Calvin–Benson–Bassham (CBB) ***, we demonstrated that formate could directly be utilized as a carbon source by yeast to synthesize endogenous amino acids. Theengineered strain achieved a one-carbon (C1) assimilation efficiency of 9.2%, which was the highest efficiency observed in the co-utilization of2G and 3G feedstocks. We applied this strategy for productions of both bulk and value-added chemicals, including ethanol, free fatty acids(FFAs), and longifolene, resulting in yield enhancements of 18.4%, 49.0%, and ~100%, respectively. The strategy demonstrated here for coutilization of 2G and 3G feedstocks sheds lights on both basic and applied research for the up-coming establishment of 3G biorefineries.