Gelatin methacryloyl granular scaffolds for localized mRNA delivery

作     者：Bruna Gregatti Carvalho Aya Nakayama Hiromi Miwa SangWon Han Lucimara Gaziola de la Torre Dino Di Carlo Junmin Lee Han-Jun Kim Ali Khademhosseini Natan Roberto de Barros 

作者机构：Terasaki Institute for Biomedical Innovation(TIBI)Los AngelesCaliforniaUSA Department of Materials and Bioprocess EngineeringSchool of Chemical EngineeringUniversity of Campinas(UNICAMP)CampinasBrazil Department of BioengineeringUniversity of California at Los Angeles(UCLA)Los AngelesCaliforniaUSA Department of BiophysicsPaulista School of MedicineFederal University of São Paulo(UNIFESP)CampinasBrazil Department of Materials Science and EngineeringPohang University of Science and Technology(POSTECH)PohangRepublic of Korea Institute for Convergence Research and Education in Advanced TechnologyYonsei UniversityIncheonRepublic of Korea College of PharmacyKorea UniversityPohangRepublic of Korea Centre for BiomaterialsCellular and Molecular Theranostics(CBCMT)Vellore Institute of Technology(VIT)VelloreIndia 

出 版 物：《Aggregate》 (聚集体（英文）)

年 卷 期：2024年第5卷第2期

页      面：386-400页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Institutes of Health,Grant/Award Numbers:HL140951,HL137193,CA257558,DK130566 Ministry of Education,Grant/Award Number:RS-2023-00240729 Korea University,Grant/Award Number:K2326671 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior,Grant/Award Numbers:2018/18523-3,2021/07057-4 Fundação de AmparoàPesquisa do Estado de São Paulo,Grant/Award Numbers:2021/07057-4,2018/18523-3,2021/11564-9 National Research Foundation of Korea,Grant/Award Number:RS-2023-00240729 

主　　题：GelMA lipid-based nanocarriers MAP scaffolds microfluidics mRNA 

摘      要：Messenger RNA(mRNA)therapy is the intracellular delivery of mRNA to produce desired therapeutic *** strategies for local mRNA delivery is still required where direct intra-articular injections are inappropriate for targeting a specific *** mRNA delivery efficiency depends on protecting nucleic acids against nuclease-mediated degradation and safe site-specific intracellular ***,novel mRNA-releasing matrices based on RGD-moiety-rich gelatin methacryloyl(GelMA)microporous annealed particle(MAP)scaffolds are *** concentration in aerogel-based microgels(μgels)produced through a microfluidic process,MAP stiffnesses,and microporosity are crucial parameters for cell adhesion,spreading,and *** being loaded with mRNA complexes,MAP scaffolds composed of 10%GelMAμgels display excellent cell viability with increasing cell infiltration,adhesion,proliferation,and gene *** intracellular delivery is achieved by the sustained release of mRNA complexes from MAP scaffolds and cell adhesion on mRNA-releasing *** findings highlight that hybrid systems can achieve efficient protein expression by delivering mRNA complexes,making them promising mRNA-releasing biomaterials for tissue engineering.