R3-MYB proteins OsTCL1 and OsTCL2 modulate seed germination via dual pathways in rice

作     者：Yong Yi Chan Lin Xueyan Peng Meishan Zhang Jiaming Wu Chunmei Meng Shengchao Ge Yunfeng Liu Yuan Su Yong Yi;Chan Lin;Xueyan Peng;Meishan Zhang;Jiaming Wu;Chunmei Meng;Shengchao Ge;Yunfeng Liu;Yuan Su

作者机构：State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of Life Science and TechnologyGuangxi UniversityNanning 530004GuangxiChina Life Sciences InstituteGuangxi Medical UniversityNanning 530021GuangxiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第6期

页      面：1752-1761页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：the National Natural Science Foundation of China(31970603) Natural Science Foundation of Guangxi province(2019GXNSFDA185001) State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources(SKLCUSA-a202008 and SKLCUSA-a01) Guangxi University-Bama Research Fund(20220006) 

主　　题：ABA Drought stress Phospholipase Salt stress Rice 

摘      要：Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germination,potentially leading to significantly impaired growth and production in *** a comprehensive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount *** the present study,we identified two R3-MYB genes in rice,namely OsTCL1 and OsTCL2,and characterized their roles in regulating seed germination under salt and drought *** with tcl1 and tcl2 mutant alleles exhibited delayed seed germination,particularly under stress *** tcl1 tcl2 double mutant showed an even more pronounced reduction in germination during initial stages of germination,thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic ***,we demonstrated that the transcript levels of several phospholipase D(PLD)genes were downregulated in the tcl1 tcl2 mutant,resulting in a decreased level of the phosphatidic acid(PA)*** of 1-butanol,a competitive substrate inhibitor of PLD-dependent production of PA,attenuated the stress response of the tcl1 tcl2 *** suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling ***,there were alterations in the expression of genes involved in abscisic acid(ABA)biosynthesis,metabolism and signaling transduction in the double *** changes affected the endogenous ABA level and ABA response,thereby influencing seed *** of both 1-butanol and ABA synthesis inhibitor sodium tungstate(Na2WO4)nearly eliminated the differences in stress response between wild type and the tcl1 tcl2 *** indicates that OsTCL1 and OsTCL2 synergistically coordinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.