Mulberry leaf supplementation inhibits skatole deposition by regulating gut microbiota and upregulating liver cytochrome P4501A1 expression in finishing pigs
作者机构：Institute of Sericulture and Tea Zhejiang Academy of Agricultural Sciences Institute of Animal Husbandry and Veterinary Science Zhejiang Academy of Agricultural Sciences
出 版 物：《Animal Nutrition》 (动物营养(英文))
年 卷 期：2024年第16卷第1期
页 面：34-44页
核心收录：
学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学]
基 金：supported by Key Scientific and Technological Grant of Zhejiang for Breeding New Agricultural Varieties Key R & D projects of Zhejiang Province Zhejiang Provincial Department of Agriculture and Rural Affairs
主 题：Pig Skatole Mulberry leaf Microbiota Cytochrome P450
摘 要：Skatole, a strong fecal odor substance, is generated through microbial degradation of tryptophan in the animal hindgut. It easily accumulates in adipose tissue and affects meat quality. In this study, the effect of mulberry leaf supplementation on skatole in finishing pigs was studied. In a 35-day trial, 20 finishing pigs (barrows and gilts) were fed with a basal diet or basal diet with 6% mulberry leaves. Growth performance of the pigs (n=10) was automatically recorded by a performance-testing feeder system and 8 pigs in each treatment were slaughtered and sampled for the remaining tests. Skatole and short-chain fatty acids were detected using HPLC and gas chromatography, respectively. Fecal microbiota were analyzed using 16S r RNA gene sequencing. The metabolomics analysis of feces and serum was performed with UHPLC-MS/MS. The major cytochrome P450 (CYP) enzymes that catalyze skatole degradation in the liver were tested by using RT-PCR and Western blot. Effects of major bioactive compounds in mulberry leaves on the CYP genes were verified in the hepatic cell line Hep G2 in an in vitro test (n=3). In finishing pigs, mulberry leaf supplementation had no significant effect on the average daily gain, average daily feed intake, and feed conversion ratio (P0.05), but reduced skatole levels in feces, serum, and backfat (P0.05), and increased acetic acid levels in feces (P=0.027). Mulberry leaf supplementation decreased the relative abundance of the skatole-producing bacteria Megasphaera and Olsenella (P0.05). Indole-3acetic acid, the intermediate that is essential for skatole production, was significantly reduced in feces by mulberry leaf supplementation (P0.05) and was positively correlated with skatole content in feces (P=0.004). In pigs treated with mulberry leaves, liver CYP1A1 expression was increased (P0.05) and was negatively correlated with skatole content in backfat (P=0.045). The in vitro test demonstrated that mulberry leaf polyphenols and polysaccharides
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